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High-content analysis of single cells directly assembled on CMOS sensor based on color imaging
High-content analysis of single cells directly assembled on CMOS sensor based on color imaging
- Source :
- Biosensorsbioelectronics. 26(4)
- Publication Year :
- 2010
-
Abstract
- A complementary metal oxide semiconductor (CMOS) image sensor was applied to high-content analysis of single cells which were assembled closely or directly onto the CMOS sensor surface. The direct assembling of cell groups on CMOS sensor surface allows large-field (6.66 mm×5.32 mm in entire active area of CMOS sensor) imaging within a second. Trypan blue-stained and non-stained cells in the same field area on the CMOS sensor were successfully distinguished as white- and blue-colored images under white LED light irradiation. Furthermore, the chemiluminescent signals of each cell were successfully visualized as blue-colored images on CMOS sensor only when HeLa cells were placed directly on the micro-lens array of the CMOS sensor. Our proposed approach will be a promising technique for real-time and high-content analysis of single cells in a large-field area based on color imaging.
- Subjects :
- Materials science
Luminescence
ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION
Biomedical Engineering
Biophysics
Color
Nanotechnology
Biosensing Techniques
Antibodies
Single-cell analysis
Hardware_INTEGRATEDCIRCUITS
Electrochemistry
Humans
Image sensor
Coloring Agents
Horseradish Peroxidase
Image Cytometry
CMOS sensor
business.industry
Detector
General Medicine
Trypan Blue
Semiconductor
Hyaluronan Receptors
CMOS
Semiconductors
High-content screening
Optoelectronics
Single-Cell Analysis
business
Biotechnology
HeLa Cells
Subjects
Details
- ISSN :
- 18734235
- Volume :
- 26
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Biosensorsbioelectronics
- Accession number :
- edsair.doi.dedup.....89e4714d22b5761586ed96922457b346