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High-content analysis of single cells directly assembled on CMOS sensor based on color imaging

High-content analysis of single cells directly assembled on CMOS sensor based on color imaging

Authors :
Yoshihiko Sunaga
Tatsuya Saeki
Tadashi Matsunaga
Tsuyoshi Tanaka
Source :
Biosensorsbioelectronics. 26(4)
Publication Year :
2010

Abstract

A complementary metal oxide semiconductor (CMOS) image sensor was applied to high-content analysis of single cells which were assembled closely or directly onto the CMOS sensor surface. The direct assembling of cell groups on CMOS sensor surface allows large-field (6.66 mm×5.32 mm in entire active area of CMOS sensor) imaging within a second. Trypan blue-stained and non-stained cells in the same field area on the CMOS sensor were successfully distinguished as white- and blue-colored images under white LED light irradiation. Furthermore, the chemiluminescent signals of each cell were successfully visualized as blue-colored images on CMOS sensor only when HeLa cells were placed directly on the micro-lens array of the CMOS sensor. Our proposed approach will be a promising technique for real-time and high-content analysis of single cells in a large-field area based on color imaging.

Details

ISSN :
18734235
Volume :
26
Issue :
4
Database :
OpenAIRE
Journal :
Biosensorsbioelectronics
Accession number :
edsair.doi.dedup.....89e4714d22b5761586ed96922457b346