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Measurement of long-chain fatty acid uptake into adipocytes
- Publication Year :
- 2014
- Publisher :
- eScholarship, University of California, 2014.
-
Abstract
- The ability of white and brown adipose tissue to efficiently take up long-chain fatty acids is key to their physiological functions in energy storage and thermogenesis respectively. Several approaches have been taken to determine uptake rates by cultured cells as well as primary adipocytes including radio- and fluorescently labeled fatty acids. In addition, the recent description of activatable bioluminescent fatty acids has opened the possibility for expanding these in vitro approaches to real-time monitoring of fatty acid uptake kinetics by adipose depots in vivo. Here we will describe some of the most useful experimental paradigms to quantitatively determine long-chain fatty acid uptake by adipocytes in vitro and provide the reader with detailed instruction on how bioluminescent probes for in vivo imaging can be synthesized and used in living mice.
- Subjects :
- Boron Compounds
Biochemistry & Molecular Biology
Fatty acid uptake
Cells
Adipose tissue
White adipose tissue
Biology
Brown adipose tissue
Article
Mice
Affordable and Clean Energy
In vivo
3T3-L1 Cells
medicine
Adipocytes
Animals
Humans
Whole Body Imaging
Long-chain fatty acids
Cells, Cultured
Metabolic and endocrine
Fluorescent Dyes
Nutrition
chemistry.chemical_classification
Cultured
Luminescent Agents
Fatty Acid Transport Proteins
Prevention
Fatty Acids
Optical Imaging
Fatty acid
Biological Transport
In vitro
medicine.anatomical_structure
Biochemistry
chemistry
Adipose Tissue
Molecular Probes
Luminescent Measurements
In vivo imaging
Fatty acid transport proteins
Biochemistry and Cell Biology
Thermogenesis
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....89fa5615e08831acf3e4e29fc0a77c2a