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HDAC1, a Histone Deacetylase, Forms a Complex with Hus1 and Rad9, Two G2/M Checkpoint Rad Proteins
- Source :
- Journal of Biological Chemistry. 275:27909-27916
- Publication Year :
- 2000
- Publisher :
- American Society for Biochemistry & Molecular Biology (ASBMB), 2000.
-
Abstract
- HDAC1 is a member of the histone deacetylase family, which plays an important role in modulating the eukaryotic chromatin structure. Numerous studies have demonstrated its involvement in transcription and in tumorigenesis. To better understand the functions and regulation of HDAC1, a yeast two-hybrid screening approach was chosen to identify novel interactions involving HDAC1. Human HDAC1 was found to interact specifically in yeast, mammalian cells, and in vitro with the human Hus1 gene product, whose Schizosaccharomyces pombe homolog has been implicated in G(2)/M checkpoint control. Both HDAC1 and Hus1 proteins localize to the nuclei. Furthermore, HDAC1 and Hus1 were found to exist in a complex with Rad9, a known Hus1-interacting factor. In addition, bioinformatics analysis of the protein sequences of Hus1, Rad1, and Rad9, three checkpoint Rad proteins that form a complex, revealed that they all contain a putative proliferating cell nuclear antigen (PCNA) fold, raising the possibility that these factors may bind to DNA in a PCNA-like ring structure. The results reported in this study strongly suggest a novel pathway involving HDAC1 in G(2)/M checkpoint control through the interaction with a functional Rad complex that may utilize a PCNA-like structure. Therefore, physically and functionally similar apparatus may function during G(2)/M checkpoint and DNA replication.
- Subjects :
- G2 Phase
Molecular Sequence Data
Mitosis
Cell Cycle Proteins
Histone Deacetylase 1
Transfection
Biochemistry
Histone Deacetylases
Protein Structure, Secondary
Proliferating Cell Nuclear Antigen
Schizosaccharomyces
Animals
Humans
Amino Acid Sequence
Cloning, Molecular
Molecular Biology
Cell Nucleus
Mammals
Genetics
Binding Sites
biology
Cell Cycle
DNA replication
Cell Biology
G2-M DNA damage checkpoint
biology.organism_classification
Recombinant Proteins
HDAC1
Chromatin
Proliferating cell nuclear antigen
Cell biology
COS Cells
Schizosaccharomyces pombe
biology.protein
Schizosaccharomyces pombe Proteins
Histone deacetylase
biological phenomena, cell phenomena, and immunity
Sequence Alignment
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 275
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....89fb0ea8640a221453ab0e9bfab99dc0