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Glycan processing in the Golgi as optimal information coding that constrains cisternal number and enzyme specificity

Authors :
Quentin Vagne
Alkesh Yadav
Garud Iyengar
Madan Rao
Pierre Sens
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Max-Planck-Gesellschaft
Laboratoire Physico-Chimie Curie [Institut Curie] (PCC)
Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne UniversiteĢ (SU)-Centre National de la Recherche Scientifique (CNRS)
Simons Centre for the Study of Living Machines
National Centre for Biological Sciences [TIFR] (NCBS)
Tata Institute for Fundamental Research (TIFR)-Tata Institute for Fundamental Research (TIFR)
Sens, Pierre
Source :
eLife. 11
Publication Year :
2022
Publisher :
eLife Sciences Publications, Ltd, 2022.

Abstract

Many proteins that undergo sequential enzymatic modification in the Golgi cisternae are displayed at the plasma membrane as cell identity markers. The modified proteins, called glycans, represent a molecular code. The fidelity of this glycan code is measured by how accurately the glycan synthesis machinery realises the desired target glycan distribution for a particular cell type and niche. In this paper, we quantitatively analyse the tradeoffs between the number of cisternae and the number and specificity of enzymes, in order to synthesize a prescribed target glycan distribution of a certain complexity. We find that to synthesize complex distributions, such as those observed in real cells, one needs to have multiple cisternae and precise enzyme partitioning in the Golgi. Additionally, for fixed number of enzymes and cisternae, there is an optimal level of specificity of enzymes that achieves the target distribution with high fidelity. Our results show how the complexity of the target glycan distribution places functional constraints on the Golgi cisternal number and enzyme specificity.<br />Comment: 30 pages

Details

ISSN :
2050084X
Volume :
11
Database :
OpenAIRE
Journal :
eLife
Accession number :
edsair.doi.dedup.....8a13f6566e152f82dde4002266237bd6
Full Text :
https://doi.org/10.7554/elife.76757