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NADPH Oxidase-Derived Reactive Oxygen Species Are Involved in the HL-60 Cell Monocytic Differentiation Induced by Isoliquiritigenin
- Source :
- Molecules, Volume 17, Issue 11, Pages 13424-13438, Molecules, Vol 17, Iss 11, Pp 13424-13438 (2012)
- Publication Year :
- 2012
- Publisher :
- MDPI, 2012.
-
Abstract
- The present study was undertaken to test the hypothesis that NADPH oxidase-derived reactive oxygen species (ROS) are involved in isoliquiritigenin (ISL)-induced monocytic differentiation in human acute promyelocytic leukemia HL-60 cells. Morphological changes, cell surface markers CD11b/CD14 and NBT-reducing ability were used to determine the differentiation of HL-60 cells, and 2,7-dichlorofluorescein (DCFH-DA) was used to detect the level of intracellular ROS. ISL-induced HL-60 cell differentiation was accompanied by an increase in the intracellular ROS levels. l-Buthionine-(S,R)-sulfoximine (BSO), N-acetyl-l-cysteine (NAC), superoxide dismutase (SOD) and 4-hydroxy-2,2,6,6-tetramethylpiperidinoxyl (Tempol) were used to interfere with ROS production. NADPH oxidase inhibitors, apocynin (APO) and diphenyleneiodonium (DPI) were used to study the role of NADPH oxidase in ISL-induced HL-60 cell differentiation. The ISL-induced HL-60 cell differentiation and intracellular ROS generation were enhanced by the oxidant BSO and inhibited by the antioxidants NAC, SOD, and tempol, and were also inhibited by the NADPH oxidase inhibitors APO and DPI. The protein and mRNA expression of the NADPH oxidase subunits gp91phox and p47phox were determined by Western blotting and RT-PCR, respectively. The levels of translation and transcription of the NADPH oxidase subunits gp91phox and p47phox increased markedly in a concentration-dependent manner. These findings suggest that NADPH oxidase plays a critical role in HL-60 cell differentiation induced by ISL and that NADPH oxidase-derived ROS is involved in the differentiation mechanism.
- Subjects :
- Cell Nucleus Shape
Cellular differentiation
Lipopolysaccharide Receptors
Pharmaceutical Science
Gene Expression
HL-60 Cells
Article
Antioxidants
Analytical Chemistry
Superoxide dismutase
lcsh:QD241-441
chemistry.chemical_compound
Chalcones
lcsh:Organic chemistry
Drug Discovery
Humans
Physical and Theoretical Chemistry
Cell Shape
chemistry.chemical_classification
reactive oxygen species
Reactive oxygen species
NADPH oxidase
Membrane Glycoproteins
biology
Cluster of differentiation
CD11 Antigens
Organic Chemistry
NADPH Oxidases
Cell Differentiation
differentiation
Molecular biology
Cell biology
isoliquiritigenin
chemistry
Chemistry (miscellaneous)
Apocynin
NADPH Oxidase 2
biology.protein
Molecular Medicine
HL-60 cell
Oxidation-Reduction
Isoliquiritigenin
Intracellular
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 17
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....8a1831bf89c481a86f5037e2768d5cd7