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Age-associated bimodal transcriptional drift reduces intergenic disparities in transcription
- Source :
- Aging (Albany NY)
- Publication Year :
- 2018
-
Abstract
- This study addressed the question of how well the quantitative transcriptome structure established in early life is maintained and how consistently it appears with increasing age, and if there is age-associated alteration of gene expression (A3GE), how much influence the Huntington's disease (HD) genotype exerts on it. We examined 285 exonic sequences of 175 genes using targeted PCR sequencing in skeletal muscle, brain, and splenic CD4+ T cells of wild-type and HD mice. In contrast to the muscle and brain, T cells exhibited large A3GE, suggesting a strong contribution to functional decline of the organism. This A3GE was markedly intensified in age-matched HD T cells, which exhibited accelerated aging as determined by reduced telomere length. Regression analysis suggested that gene expression levels change at a rate of approximately 3% per month with age. We found a bimodal relationship in A3GE in T cells in that weakly expressed genes in young mice were increasingly transcribed in older animals whereas highly expressed genes in the young were decreasingly expressed with age. This bimodal transcriptional drift in the T cell transcriptome data causes the differences in transcription rate between genes to progressively reduce with age.
- Subjects :
- 0301 basic medicine
CD4-Positive T-Lymphocytes
Aging
Transcription, Genetic
T cell
T cells
Gene Expression
Mice, Transgenic
Biology
Transcriptome
03 medical and health sciences
Mice
Huntington's disease
Transcription (biology)
Gene expression
medicine
Animals
Humans
Gene
and transcription
Huntingtin Protein
Skeletal muscle
Cell Biology
multiplex PCR
medicine.disease
Molecular biology
Telomere
030104 developmental biology
medicine.anatomical_structure
Huntington Disease
Research Paper
Huntington’s disease
Subjects
Details
- ISSN :
- 19454589
- Volume :
- 10
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Aging
- Accession number :
- edsair.doi.dedup.....8a31cf1e6d9bd5fb54144c889e3b4146