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TEMPORAL-SPATIAL EXPRESSION OF TWOPARACENTROTUS LIVIDUSCELL SURFACE PROTEINS
- Source :
- Cell Biology International. 22:305-311
- Publication Year :
- 1998
- Publisher :
- Wiley, 1998.
-
Abstract
- The temporal expression of two cell surface proteins, called BEP1 and BEP4, during Paracentrosus lividus embryonic development was studied. These proteins are found in both monomeric and dimeric forms in egg and embryos and we have established that their specific form is related to their being in the cytoplasm or on the cell surface. The spatial distribution of BEP1 and BEP4 proteins in eggs and embryos was established by whole mount immunohistochemistry. These proteins are located in the animal part of unfertilized and fertilized eggs; thereafter they are much less represented in structures derived from the vegetal cells of the embryo such as the micromeres of the 16 cell stage, the primary mesenchyme of blastula and the gut of gastrula. At the prism stage BEP1 and BEP4 proteins are present to some ectodermal parts and thereafter, at the pluteus stage, to the oral region.
- Subjects :
- Time Factors
animal structures
Recombinant Fusion Proteins
Mesenchyme
Blotting, Western
Immunoblotting
Biology
Paracentrotus lividus
medicine
Animals
Tissue Distribution
Pluteus
Embryogenesis
Membrane Proteins
Embryo
Cell Biology
General Medicine
biology.organism_classification
Blastula
Immunohistochemistry
Precipitin Tests
Molecular biology
Cell biology
Gastrulation
medicine.anatomical_structure
Cytoplasm
Sea Urchins
embryonic structures
Subjects
Details
- ISSN :
- 10656995
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Cell Biology International
- Accession number :
- edsair.doi.dedup.....8a4091a1ea9e22b738ed47f9a0bc8845