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OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice
- Source :
- Reproductive Biology and Endocrinology : RB&E, Reproductive Biology and Endocrinology, Vol 19, Iss 1, Pp 1-8 (2021)
- Publication Year :
- 2021
- Publisher :
- BioMed Central, 2021.
-
Abstract
- Otogelin-like protein (encoded by Otogl) was highly structural similar to the gelforming mucin proteins. Although human OTOG mutations have been linked to deafness, the biological function of OTOGL in male germ cell development remains enigmatic. In screening 336 patients with non-obstructive azoospermia (NOA), OTOGL displays the high mutant ratio (13.99 %). Then, we examined the expression of OTOGL in developing mouse testes. Otogl mRNA and protein are continually expressed in postnatal developing testes from postnatal day 0 (P0) testes to P21 testes exhibiting a decreased trend with the age growth. We thus generated a global Otogl knockout mouse (KO) model using the CRISPR/Cas9 technology; however, Otogl KO mice displayed normal development and fertility. Further histological analysis of Otogl knockout mouse testes revealed that all types of spermatogenic cells are present in Otogl KO seminiferous tubules. Together, our study suggested that OTOGL is nonessential for male germ cell development and spermatogenesis.
- Subjects :
- 0301 basic medicine
QH471-489
Mutant
Biology
Andrology
03 medical and health sciences
0302 clinical medicine
Endocrinology
medicine
CRISPR
Spermatogenesis
CRISPR/Cas9
Azoospermia
Messenger RNA
Reproduction
Research
Mucin
Obstetrics and Gynecology
OTOGL
Gynecology and obstetrics
medicine.disease
030104 developmental biology
medicine.anatomical_structure
Fertility
Knockout mouse model
Reproductive Medicine
030220 oncology & carcinogenesis
Knockout mouse
RG1-991
Germ cell
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 14777827
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Reproductive Biology and Endocrinology : RB&E
- Accession number :
- edsair.doi.dedup.....8a7a2a17144af3358379eb9b1c8a4b7f