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Heritability of fat distributions in male mice from the founder strains of the Diversity Outbred mouse population
- Source :
- G3: Genes|Genomes|Genetics, G3: Genes, Genomes, Genetics, Vol 11, Iss 5 (2021)
- Publication Year :
- 2021
- Publisher :
- Oxford University Press, 2021.
-
Abstract
- Specific fat distributions are risk factors for complex diseases, including coronary heart disease and obstructive sleep apnea. To demonstrate the utility of high-diversity mouse models for elucidating genetic associations, we describe the phenotyping and heritability of fat distributions within the five classical inbred and three wild-derived founder mouse strains of the Collaborative Cross and Diversity Outbred mice. Measurements of subcutaneous and internal fat volumes in the abdomen, thorax and neck, and fat volumes in the tongue and pericardium were obtained using magnetic resonance imaging in male mice from the A/J (n = 12), C57BL/6J (n = 17), 129S1/SvlmJ (n = 12), NOD/LtJ (n = 14), NZO/HILtJ (n = 12), CAST/EiJ (n = 14), PWK/PhJ (n = 12), and WSB/EiJ (n = 15) strains. Phenotypes were compared across strains using analysis of variance and heritability estimated as the proportion of phenotypic variability attributable to strain. Heritability ranged from 44 to 91% across traits, including >70% heritability of tongue fat. A majority of heritability estimates remained significant controlling for body weight, suggesting genetic influences independent of general obesity. Principal components analysis supports genetic influences on overall obesity and specific to increased pericardial and intra-neck fat. Thus, among the founder strains of the Collaborative Cross and Diversity Outbred mice, we observed significant heritability of subcutaneous and internal fat volumes in the neck, thorax and abdomen, pericardial fat volume and tongue fat volume, consistent with genetic architecture playing an important role in explaining trait variability. Findings pave the way for studies utilizing high-diversity mouse models to identify genes affecting fat distributions and, in turn, influencing risk for associated complex disorders.
- Subjects :
- AcademicSubjects/SCI01140
Collaborative Cross Mice
Male
mice
AcademicSubjects/SCI00010
Population
Physiology
Mice, Inbred Strains
QH426-470
Biology
heritability
AcademicSubjects/SCI01180
Collaborative Cross
Diversity Outbred
03 medical and health sciences
0302 clinical medicine
Tongue
Mice, Inbred NOD
Genetics
medicine
magnetic resonance imaging
Animals
education
Molecular Biology
Genetics (clinical)
030304 developmental biology
Investigation
0303 health sciences
education.field_of_study
Strain (biology)
Heritability
medicine.disease
Obesity
Genetic architecture
Mice, Inbred C57BL
medicine.anatomical_structure
Phenotype
fat distributions
Abdomen
AcademicSubjects/SCI00960
Analysis of variance
founder strains
human activities
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 21601836
- Volume :
- 11
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- G3: Genes|Genomes|Genetics
- Accession number :
- edsair.doi.dedup.....8ad9d52b3813e57b7ffeaa0169ead88c