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A retrospective overview of enterovirus infection diagnosis and molecular epidemiology in the public hospitals of Marseille, France (1985-2005)
- Source :
- PLoS ONE, Vol 6, Iss 3, p e18022 (2011), PLoS ONE
- Publication Year :
- 2011
- Publisher :
- Public Library of Science (PLoS), 2011.
-
Abstract
- Human enteroviruses (HEV) are frequent human pathogens and, associated in particular with large outbreaks of aseptic meningitis. Here, we have compiled a database of clinical HEV isolates from the Public Hospitals of Marseille, from 1985 to 2005. Amongst 654 isolates that could be characterized by complete sequencing of the VP1 gene, 98% belonged to species HEV-B; the most frequently isolated serotypes were Echovirus E30, E11, E7, E6 and E4. The high incidence of E30 and the recent emergence of E13 are consistent with reports worldwide and peak HEV isolation occurred mostly in the late spring and summer months. The proportion of echoviruses has decreased across the years, while that of coxsackieviruses has increased. Stool (the most frequent sample type) allowed detection of all identified serotypes. MRC5 (Human lung fibroblasts) cell line was the most conducive cell line for HEV isolation (84.9% of 10 most common serotype isolates, 96.3% in association with BGM (Buffalo green monkey kidney cells)). Previous seroneutralization-based serotype identification demonstrated 55.4% accuracy when compared with molecular VP1 analysis. Our analysis of a large number of clinical strains over 20 years reinforced the validity of VP1 serotyping and showed that comparative p-distance scores can be coupled with phylogenetic analysis to provide non-ambiguous serotype identification. Phylogenetic analysis in the VP1, 2C and 3D regions also provided evidence for recombination events amongst clinical isolates. In particular, it identified isolates with dissimilar VP1 but almost identical nonstructural regions.
- Subjects :
- Serotype
Viral Diseases
Echovirus
Epidemiology
viruses
medicine.disease_cause
Emerging Viral Diseases
Phylogeny
Enterovirus
Recombination, Genetic
Molecular Epidemiology
Multidisciplinary
Phylogenetic tree
Reverse Transcriptase Polymerase Chain Reaction
Aseptic meningitis
virus diseases
Phylogenetics
Infectious Diseases
Medical Microbiology
Medicine
France
Research Article
Clinical Research Design
Science
Biology
Coxsackievirus
Microbiology
Viral Evolution
Cell Line
Evolution, Molecular
Neutralization Tests
Virology
Enterovirus Infections
medicine
Animals
Humans
Evolutionary Systematics
Serotyping
Retrospective Studies
Evolutionary Biology
Molecular epidemiology
Hospitals, Public
Computational Biology
Outbreak
medicine.disease
biology.organism_classification
Viral Disease Diagnosis
Viral Classification
Emerging Infectious Diseases
Enterovirus Infection
Population Genetics
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....8b1a2f196e395f80085ee8b7d32d2891