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CD161 is a marker of all human IL-17-producing T-cell subsets and is induced by RORC
- Source :
- European journal of immunology. 40(8)
- Publication Year :
- 2010
-
Abstract
- We have previously shown that human Th17 lymphocytes are characterized by the selective expression of IL-23 receptor (IL-23R), CCR6, CD161, and the transcription factor retinoic acid-related orphan receptor C (RORC), and originate from a CD161(+)CD4(+) naive T-cell precursor in response to the combined activity of IL-1β and IL-23. We show here that not only CD4(+)TCRαβ(+), but also CD8(+)TCRαβ(+), CD4(-)CD8(-) TCRαβ(+), and CD4(-)CD8(-) TCRγδ(+) circulating lymphocytes that produce IL-17 express the distinctive marker CD161 on their surface. In addition, we demonstrate that CD161 expression identifies CD8(+) and CD4(-)CD8(-) umbilical cord blood T cells that already express RORC and IL-23R mRNA and that can be induced to differentiate into IL-17-producing cells in the presence of IL-1β and IL-23. Finally, we provide evidence that umbilical cord blood naive CD4(+)CD161(-) T cells, upon lentivirus-mediated transduction with RORC2 can acquire the ability to express IL-23R, IL-1RI, and CD161, as well as to produce IL-17. Taken together, these data allow to conclude that T-cell subsets able to produce IL-17, as well as precursors of IL-17-producing T cells, exhibit surface expression of CD161, and that this feature is at least in part RORC2-dependent.
- Subjects :
- T cell
Cellular differentiation
CD8 Antigens
Receptors, Antigen, T-Cell, alpha-beta
Immunology
Interleukin-1beta
C-C chemokine receptor type 6
Biology
Lymphocyte Activation
Interleukin-23
Antigen
RAR-related orphan receptor gamma
T-Lymphocyte Subsets
medicine
Immunology and Allergy
Humans
Transgenes
Receptor
Cells, Cultured
Orphan receptor
Interleukin-17
Cell Differentiation
Receptors, Antigen, T-Cell, gamma-delta
T-Lymphocytes, Helper-Inducer
Nuclear Receptor Subfamily 1, Group F, Member 3
Molecular biology
medicine.anatomical_structure
CD4 Antigens
CD8
Biomarkers
NK Cell Lectin-Like Receptor Subfamily B
Subjects
Details
- ISSN :
- 15214141
- Volume :
- 40
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- European journal of immunology
- Accession number :
- edsair.doi.dedup.....8b2b053a54bc30b006d942e21294442f