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Cytosol protein regulation in H295R steroidogenesis model induced by the zearalenone metabolites, α- and β-zearalenol
Cytosol protein regulation in H295R steroidogenesis model induced by the zearalenone metabolites, α- and β-zearalenol
- Source :
- Toxicon. 59:17-24
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- α- and β-zearalenol (α-ZOL and β-ZOL, respectively) are metabolites of the mycotoxin zearalenone (ZEN). All three individual mycotoxins have shown to be biological active i.e. being estrogenic and able to stimulate cellular proliferation albeit at different strengths. In this work, cytosol protein expression was determined by using stable-isotope labelling by amino acids in cell culture (SILAC) upon exposure of α-ZOL and β-ZOL to the steroidogenesis cell model H295R. A total of 14 and 5 individual proteins were found to be significantly regulated by α-ZOL and β-ZOL, respectively. Interestingly, there were no common protein regulations by the metabolites or the parent mycotoxin ZEN. Furthermore, the regulated proteins were assigned to networks and groups of actions that also differed from one another suggesting that the three individual mycotoxins may have unique biological activities.
- Subjects :
- chemistry.chemical_classification
Quantitative proteomics
Proteins
Biology
Toxicology
Cell Line
Amino acid
Cytosol
chemistry.chemical_compound
Gene Expression Regulation
Biochemistry
chemistry
Protein regulation
Labelling
Stable isotope labeling by amino acids in cell culture
Humans
Zearalenone
Zeranol
Steroids
Protein Interaction Maps
Mycotoxin
Subjects
Details
- ISSN :
- 00410101
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Toxicon
- Accession number :
- edsair.doi.dedup.....8b3aa82823d94a0dfd691a550f12d530
- Full Text :
- https://doi.org/10.1016/j.toxicon.2011.10.007