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Cytosol protein regulation in H295R steroidogenesis model induced by the zearalenone metabolites, α- and β-zearalenol

Cytosol protein regulation in H295R steroidogenesis model induced by the zearalenone metabolites, α- and β-zearalenol

Authors :
Silvio Uhlig
Erik Ropstad
Øyvind L. Busk
Caroline Frizzell
Steven Verhaegen
Lisa Connolly
Morten Sørlie
Source :
Toxicon. 59:17-24
Publication Year :
2012
Publisher :
Elsevier BV, 2012.

Abstract

α- and β-zearalenol (α-ZOL and β-ZOL, respectively) are metabolites of the mycotoxin zearalenone (ZEN). All three individual mycotoxins have shown to be biological active i.e. being estrogenic and able to stimulate cellular proliferation albeit at different strengths. In this work, cytosol protein expression was determined by using stable-isotope labelling by amino acids in cell culture (SILAC) upon exposure of α-ZOL and β-ZOL to the steroidogenesis cell model H295R. A total of 14 and 5 individual proteins were found to be significantly regulated by α-ZOL and β-ZOL, respectively. Interestingly, there were no common protein regulations by the metabolites or the parent mycotoxin ZEN. Furthermore, the regulated proteins were assigned to networks and groups of actions that also differed from one another suggesting that the three individual mycotoxins may have unique biological activities.

Details

ISSN :
00410101
Volume :
59
Database :
OpenAIRE
Journal :
Toxicon
Accession number :
edsair.doi.dedup.....8b3aa82823d94a0dfd691a550f12d530
Full Text :
https://doi.org/10.1016/j.toxicon.2011.10.007