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Human plasma protein corona decreases the toxicity of pillar-layer metal organic framework
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- This scenario was designed to investigate the protein corona pattern on the pillar-layer surface of a Cu-based metal–organic framework (MOF) in human plasma. The [Cu(L)(L/)].1.3DMA (MOF-1) {L = 4, 4/-bipyridine and L/ = 5-aminoisophthalic acid}, was synthesized through the sonochemical irradiation approach as well as characterized by various techniques like scanning electron microscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction and single-crystal X-ray diffraction. The space group was determined to be an orthorhombic space group (Pbam) by single-crystal X-ray diffraction. Single-crystal X-ray analyses on MOF-1 showed that Cu+2 ion was 6-coordinated. Besides, to study and clarify interactions between MOFs and biological milieu, human whole blood plasma was selected as a model. Fluorescence spectroscopy and SDS-PAGE techniques were employed to explore quantitative and qualitative in situ characterization of protein corona as well. Furthermore, cell viability in a cancerous cell lines was evaluated by MTT assay in the presence and absence of the corona. The results from SDS-PAGE illustrated that the most adsorbed quantity among plasma proteins belongs to fibrinogen (α, β and γ chains), and this protein showed the maximum frequency on the MOF-1s surface, so the possible interactions of MOF-1s with fibrinogen also studied using fluorescence spectroscopy and corresponding data were plotted. According to the obtained data from MTT assay, these structures have concentration-dependent toxicity. In brief, based on the obtained data in the current study, the designed MOF can be introduced as a new desirable carrier for drug/gen delivery after further prerequisite assessments.
- Subjects :
- 0301 basic medicine
Materials science
Biomaterials - proteins
Scanning electron microscope
Analytical chemistry
lcsh:Medicine
Infrared spectroscopy
Breast Neoplasms
Serum Albumin, Human
Protein Corona
Article
Fluorescence spectroscopy
Ion
Biomaterials
03 medical and health sciences
Drug Delivery Systems
0302 clinical medicine
Humans
MTT assay
lcsh:Science
Metal-Organic Frameworks
Cell Proliferation
Multidisciplinary
fungi
lcsh:R
030104 developmental biology
MCF-7 Cells
Female
Serum Globulins
lcsh:Q
Orthorhombic crystal system
030217 neurology & neurosurgery
Powder diffraction
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....8b74fd1dce50a5bf972dd5a7f6a29f7f