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Insights into Streptococcus agalactiae PI-2b pilus biosynthesis and role in adherence to host cells

Authors :
Cherry Gao
Patrick Trieu-Cuot
Claire Poyart
Bruno Périchon
Julie Guignot
Shaynoor Dramsi
Noémi Szili
Biologie des Bactéries pathogènes à Gram-positif - Biology of Gram-Positive Pathogens (BBPG+)
Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
Barrières et Pathogènes
[Institut Cochin] Departement Infection, immunité, inflammation
Institut Cochin (IC UM3 (UMR 8104 / U1016))
Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Cochin (IC UM3 (UMR 8104 / U1016))
Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Centre national de Référence des Streptocoques (CNR)
Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)
This work was supported by the DIM Malinf from the Conseil Régional d’Ile-de-France (Grant DIM130065) to CP and SD and the French Government's Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' Grant ANR-10-LABX-62-IBEID. NS was recipient of a doctoral fellowship from the DIM Malinf.
ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Cochin (IC UM3 (UMR 8104 / U1016))
Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Source :
Microbes and Infection, Microbes and Infection, Elsevier, 2019, 21 (2), pp.99-103. ⟨10.1016/j.micinf.2018.10.004⟩, Microbes and Infection, 2019, 21 (2), pp.99-103. ⟨10.1016/j.micinf.2018.10.004⟩
Publication Year :
2019
Publisher :
HAL CCSD, 2019.

Abstract

International audience; The core PI-2b pilus present in "hypervirulent" ST-17 Streptococcus agalactiae strains consists of three pilin subunits (Spb1, Ap1 and Ap2) assembled by sortase SrtC1 and cell-wall anchored by Srt2. Spb1 was shown to be the major pilin and Ap2 the anchor pilin. Ap1 is a putative adhesin. Two additional genes, orf and lep, are part of this operon. The contribution of Lep and Ap1 to the biogenesis of the PI-2b pilus was investigated. Concerning the role of PI-2b, we found that higher PI-2b expression resulted in higher adherence to human brain endothelial cells and higher phagocytosis by human THP1 macrophages.

Subjects

Subjects :
0301 basic medicine
Group B Streptococcus
MESH: Streptococcus agalactiae / physiology
Operon
medicine.disease_cause
Bacterial Adhesion
Pilus
MESH: Adhesins, Bacterial / genetics
S. agalactiae
Cell Wall
Sortase
MESH: Phagocytosis
MESH: Endothelial Cells / microbiology
MESH: Humans Macrophages / microbiology
biology
Infectious Diseases
MESH: Adhesins, Bacterial / metabolism
MESH: Streptococcus agalactiae / pathogenicity
030106 microbiology
Immunology
education
GBS
Microbiology
Streptococcus agalactiae
THP-1 phagocytosis
03 medical and health sciences
Phagocytosis
Streptococcal Infections
medicine
PI-2b pilus
Humans
MESH: Streptococcal Infections / microbiology
MESH: Bacterial Adhesion
Adhesins, Bacterial
Gene
Macrophages
MESH: Fimbriae, Bacterial / metabolism
Endothelial Cells
MESH: Cell Wall / metabolism
biochemical phenomena, metabolism, and nutrition
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Streptococcus agalactiae / genetics
MESH: Operon / genetics
Bacterial adhesin
030104 developmental biology
Fimbriae, Bacterial
Pilin
biology.protein
bacteria
Biogenesis

Details

Language :
English
ISSN :
12864579
Database :
OpenAIRE
Journal :
Microbes and Infection, Microbes and Infection, Elsevier, 2019, 21 (2), pp.99-103. ⟨10.1016/j.micinf.2018.10.004⟩, Microbes and Infection, 2019, 21 (2), pp.99-103. ⟨10.1016/j.micinf.2018.10.004⟩
Accession number :
edsair.doi.dedup.....8b7c425b2b9077d1b11a6b168b081969

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