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Insights into Streptococcus agalactiae PI-2b pilus biosynthesis and role in adherence to host cells
- Source :
- Microbes and Infection, Microbes and Infection, Elsevier, 2019, 21 (2), pp.99-103. ⟨10.1016/j.micinf.2018.10.004⟩, Microbes and Infection, 2019, 21 (2), pp.99-103. ⟨10.1016/j.micinf.2018.10.004⟩
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- International audience; The core PI-2b pilus present in "hypervirulent" ST-17 Streptococcus agalactiae strains consists of three pilin subunits (Spb1, Ap1 and Ap2) assembled by sortase SrtC1 and cell-wall anchored by Srt2. Spb1 was shown to be the major pilin and Ap2 the anchor pilin. Ap1 is a putative adhesin. Two additional genes, orf and lep, are part of this operon. The contribution of Lep and Ap1 to the biogenesis of the PI-2b pilus was investigated. Concerning the role of PI-2b, we found that higher PI-2b expression resulted in higher adherence to human brain endothelial cells and higher phagocytosis by human THP1 macrophages.
- Subjects :
- 0301 basic medicine
Group B Streptococcus
MESH: Streptococcus agalactiae / physiology
Operon
medicine.disease_cause
Bacterial Adhesion
Pilus
MESH: Adhesins, Bacterial / genetics
S. agalactiae
Cell Wall
Sortase
MESH: Phagocytosis
MESH: Endothelial Cells / microbiology
MESH: Humans Macrophages / microbiology
biology
Infectious Diseases
MESH: Adhesins, Bacterial / metabolism
MESH: Streptococcus agalactiae / pathogenicity
030106 microbiology
Immunology
education
GBS
Microbiology
Streptococcus agalactiae
THP-1 phagocytosis
03 medical and health sciences
Phagocytosis
Streptococcal Infections
medicine
PI-2b pilus
Humans
MESH: Streptococcal Infections / microbiology
MESH: Bacterial Adhesion
Adhesins, Bacterial
Gene
Macrophages
MESH: Fimbriae, Bacterial / metabolism
Endothelial Cells
MESH: Cell Wall / metabolism
biochemical phenomena, metabolism, and nutrition
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Streptococcus agalactiae / genetics
MESH: Operon / genetics
Bacterial adhesin
030104 developmental biology
Fimbriae, Bacterial
Pilin
biology.protein
bacteria
Biogenesis
Subjects
Details
- Language :
- English
- ISSN :
- 12864579
- Database :
- OpenAIRE
- Journal :
- Microbes and Infection, Microbes and Infection, Elsevier, 2019, 21 (2), pp.99-103. ⟨10.1016/j.micinf.2018.10.004⟩, Microbes and Infection, 2019, 21 (2), pp.99-103. ⟨10.1016/j.micinf.2018.10.004⟩
- Accession number :
- edsair.doi.dedup.....8b7c425b2b9077d1b11a6b168b081969