Back to Search Start Over

Characterization of internal ribosome entry sites according to secondary structure analysis to classify Border Disease virus strains

Authors :
Pasquale Turno
Rebecca Strong
Falko Steinbach
Nicola Decaro
Massimo Giangaspero
Lorenzo Domenis
Claudio Apicella
Canio Buonavoglia
Xavier Bailly
Pietro Gargano
Faculty of Veterinary Medicine
Universita degli studi di Teramo - University of Teramo [Italie] (UNITE)
Mammalian Virology Group
Virology Department
Animal and Plant Health Agency [Addlestone, UK] (APHA)-Animal and Plant Health Agency [Addlestone, UK] (APHA)
Università degli studi di Bari Aldo Moro (UNIBA)
Centro di Referenza Nazionale per le Malattie degli Animali, Selvatici ( (CeRMAS))
Istituto Zooprofilattico Sperimentale del Piemonte
Liguria e Valle d'Aosta-Liguria e Valle d'Aosta
Istituto Zooprofilattico Sperimentale del Mezzogiorno
Unité Mixte de Recherche d'Épidémiologie des maladies Animales et zoonotiques (UMR EPIA)
VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Directorate General of Veterinary Public Health, Food and Nutrition
Ministry of Health
Department for Environment, Food & Rural Affairs (DEFRA) ED1000 1100
Source :
Journal of Virological Methods, Journal of Virological Methods, Elsevier, 2020, 275, pp.113704. ⟨10.1016/j.jviromet.2019.113704⟩
Publication Year :
2019
Publisher :
Elsevier, 2019.

Abstract

Applying palindromic nucleotide substitutions (PNS) method, variable loci of the internal ribosome entry site (IRES) secondary structure in the 5' untranslated region (UTR) of Border disease virus sequences were analysed allowing their allocation into ten IRES classes within the species. Sequence characteristics of Turkish and Chinese strains were highly divergent from other genogroups, indicating geographic segregation and micro-evolutive steps within the species. Observed heterogeneity in the BDV species has to be considered for potential implications on diagnostic tests, control and preventive measures.

Details

Language :
English
ISSN :
01660934
Database :
OpenAIRE
Journal :
Journal of Virological Methods, Journal of Virological Methods, Elsevier, 2020, 275, pp.113704. ⟨10.1016/j.jviromet.2019.113704⟩
Accession number :
edsair.doi.dedup.....8bac7e7202e0d5fa323b52e1713ce80e
Full Text :
https://doi.org/10.1016/j.jviromet.2019.113704⟩