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High-mobility group box (TOX) antibody a useful tool for the identification of B and T cell subpopulations
- Source :
- Repisalud, Instituto de Salud Carlos III (ISCIII), PLoS ONE, Vol 15, Iss 2, p e0229743 (2020), PLoS ONE
- Publication Year :
- 2020
- Publisher :
- BMC, 2020.
-
Abstract
- Thymocyte selection-associated high-mobility group box (TOX) is a DNA-binding factor that is able to regulate transcription by modifying local chromatin structure and modulating the formation of multi-protein complexes. TOX has multiple roles in the development of the adaptive immune system including development of CD4 T cells, NK cells and lymph node organogenesis. However very few antibodies recognizing this molecule have been reported and no extensive study of the expression of TOX in reactive and neoplastic lymphoid tissue has been performed to date. In the present study, we have investigated TOX expression in normal and neoplastic lymphoid tissues using a novel rat monoclonal antibody that recognizes its target molecule in paraffin-embedded tissue sections. A large series of normal tissues and B- and T-cell lymphomas was studied, using whole sections and tissue microarrays. We found that the majority of precursor B/T lymphoblastic, follicular and diffuse large B-cell lymphomas, nodular lymphocyte-predominant Hodgkin lymphomas and angioimmunoblastic T-cell lymphomas strongly expressed the TOX protein. Burkitt and mantle cell lymphomas showed TOX expression in a small percentage of cases. TOX was not found in the majority of chronic lymphocytic leukemia, myelomas, marginal zone lymphomas and classical Hodgkin lymphomas. In conclusion, we describe for the first time the expression of TOX in normal and neoplastic lymphoid tissues. The co-expression of TOX and PD-1 identified in normal and neoplastic T cells is consistent with recent studies identifying TOX as a critical regulator of T-cell exhaustion and a potential immunotherapy target. Its differential expression may be of diagnostic relevance in the differential diagnosis of follicular lymphoma, the identification of the phenotype of diffuse large B-cell lymphoma and the recognition of peripheral T-cell lymphoma with a follicular helper T phenotype. This work was supported by grants from the Plan Nacional de I+D+I, co-financed by the ISCIII-Subdireccion General de Evaluacion and the Fondo Europeo de Desarrollo Regional (FEDER), CIBERONC -CB16/12/00291 (MAP), and Programs of R&D activities among research groups of the Community of Madrid in Biomedicine (B2017/BMD-3778) (MAP, GR, JFGG). All funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Sí
- Subjects :
- 0301 basic medicine
Male
B Cells
Physiology
Chronic lymphocytic leukemia
Protein Expression
Follicular lymphoma
Gene Expression
Hematologic Cancers and Related Disorders
White Blood Cells
Antibodies, Monoclonal, Murine-Derived
Mice
fluids and secretions
0302 clinical medicine
Animal Cells
immune system diseases
Antibody Specificity
T-Lymphocyte Subsets
Immune Physiology
hemic and lymphatic diseases
Medicine and Health Sciences
Lymph node
Staining
Multidisciplinary
T Cells
High Mobility Group Proteins
Cell Staining
Hematology
Marginal zone
Thymocyte
medicine.anatomical_structure
Lymphatic system
Oncology
030220 oncology & carcinogenesis
Medicine
Lymphomas
Female
Cellular Types
Anatomy
Research Article
Lymphoma, B-Cell
Lymphoid Tissue
Immune Cells
Science
T cell
Immunology
B-Lymphocyte Subsets
Biology
Research and Analysis Methods
Lymphoma, T-Cell
Lymphatic System
03 medical and health sciences
Cell Line, Tumor
Gene Expression and Vector Techniques
medicine
Animals
Humans
RNA, Messenger
Rats, Wistar
Antibody-Producing Cells
Molecular Biology Techniques
Immunohistochemistry Techniques
Molecular Biology
Molecular Biology Assays and Analysis Techniques
Blood Cells
Biology and Life Sciences
Cancers and Neoplasms
Cell Biology
medicine.disease
Lymphoma
Rats
Histochemistry and Cytochemistry Techniques
Mice, Inbred C57BL
030104 developmental biology
Specimen Preparation and Treatment
Immunologic Techniques
Cancer research
bacteria
Lymph Nodes
Spleen
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Repisalud, Instituto de Salud Carlos III (ISCIII), PLoS ONE, Vol 15, Iss 2, p e0229743 (2020), PLoS ONE
- Accession number :
- edsair.doi.dedup.....8bac814e827b957652eccd4363714385