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A comparative study on chitosan nanoparticle synthesis methodologies for application in aquaculture through toxicity studies
- Source :
- IET Nanobiotechnology, Vol 15, Iss 4, Pp 418-426 (2021), IET Nanobiotechnology
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Chitosan nanoparticles (CSNPs) have been recently used for various applications in aquaculture, especially as drug carriers. The aim of this study was to synthesise and investigate a superlative method of CSNP synthesis for application in aquaculture through aquaculture‐based toxicology screening methods. Two different methods were analysed: the first a direct ionic gelation method (A) and the other involving a low‐molecular‐weight chitosan microparticle intermediate method (B). Dynamic light scattering characterisation revealed that the CSNP particle sizes were 192.7 ± 11.8 and 22.9 nm from methods A and B, respectively. The LC50 values for brine shrimp toxicity were found to be 1.51 and 0.02 ppt in 24 h for methods A and B, respectively. Acute toxicity studies in Litopenaeus vannamei rendered LC50 values of 3235.94 and 2884.03 ppt in 24 h for methods A and B, respectively. Zebrafish toxicity studies revealed mortality rates of 21.67% and 55% at 20 mg/L concentration for methods A and B, respectively, with an increased expression of intracellular reactive oxygen species in method B. From these findings, it can be concluded that a comparatively reduced toxicity of CSNPs derived from ionic gelation method makes it more appropriate for application in aquaculture.
- Subjects :
- Litopenaeus
Brine shrimp
Aquaculture
Article
Chitosan
chemistry.chemical_compound
Animals
Electrical and Electronic Engineering
Microparticle
Zebrafish
Drug Carriers
Chromatography
biology
business.industry
biology.organism_classification
Acute toxicity
Electronic, Optical and Magnetic Materials
Original Research Paper
chemistry
Toxicity
Nanoparticles
business
Drug carrier
TP248.13-248.65
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 17518741
- Volume :
- 15
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- IET Nanobiotechnology
- Accession number :
- edsair.doi.dedup.....8bcb5f0bef95aeed7e4aab0a54083ef2