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Purified β‐glucans from the Shiitake mushroom ameliorates antibiotic‐resistant Klebsiella pneumoniae ‐induced pulmonary sepsis
- Source :
- Letters in Applied Microbiology.
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- Bacterial infection remains the main cause of acute respiratory distress syndrome and is a leading cause of death and disability in critically ill patients Here we report on the use of purified beta-glucan (lentinan) extracts from Lentinus edodes (Shiitake) mushroom that can reduce infection by a multidrug-resistant clinical isolate of Klebsiella pneumoniae in a rodent pneumonia model, likely through immunomodulation Adult male Sprague-Dawley rats were subjected to intra-tracheal administration of K pneumoniae to induce pulmonary sepsis and randomized to three groups;vehicle control (Vehicle, n = 12), commercial lentinan (CL, n = 8) or in-house extracted lentinan (IHL, n = 8) were administered intravenously 1 h postinfection Physiological parameters and blood gas analysis were measured, bacterial counts from bronchoalveolar-lavage (BAL) were determined, along with differential staining of white cells and measurement of protein concentration in BAL 48 h after pneumonia induction Use of IHL extract significantly decreased BAL CFU counts Both CL and IHL extractions reduced protein concentration in BAL Use of IHL resulted in an improvement in physiological parameters compared to controls and CL In conclusion, administration of lentinan to treat sepsis-induced lung injury appears safe and effective and may exert its effects in an immunomodulatory manner
- Subjects :
- Lung Diseases
Male
0106 biological sciences
beta-Glucans
Klebsiella pneumoniae
Lentinan
Shiitake Mushrooms
Lung injury
01 natural sciences
Applied Microbiology and Biotechnology
Microbiology
Rats, Sprague-Dawley
Sepsis
03 medical and health sciences
chemistry.chemical_compound
Antibiotic resistance
010608 biotechnology
Drug Resistance, Bacterial
medicine
Animals
Humans
0303 health sciences
Mushroom
biology
Plant Extracts
030306 microbiology
business.industry
medicine.disease
biology.organism_classification
Anti-Bacterial Agents
Rats
respiratory tract diseases
Pneumonia
chemistry
Lentinus
business
Subjects
Details
- ISSN :
- 1472765X and 02668254
- Database :
- OpenAIRE
- Journal :
- Letters in Applied Microbiology
- Accession number :
- edsair.doi.dedup.....8bd9c6f7ea5c7ef01a22c435ca82f3b0