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Fat Grafting in Burn Scar Alleviates Neuropathic Pain via Anti-Inflammation Effect in Scar and Spinal Cord
- Source :
- PLoS ONE, PLoS ONE, Vol 10, Iss 9, p e0137563 (2015)
- Publication Year :
- 2015
-
Abstract
- Burn-induced neuropathic pain is complex, and fat grafting has reportedly improved neuropathic pain. However, the mechanism of fat grafting in improving neuropathic pain is unclear. Previous investigations have found that neuroinflammation causes neuropathic pain, and anti-inflammatory targeting may provide potential therapeutic opportunities in neuropathic pain. We hypothesized that fat grafting in burn scars improves the neuropathic pain through anti-inflammation. Burn-induced scar pain was confirmed using a mechanical response test 4 weeks after burn injuries, and autologous fat grafting in the scar area was performed simultaneously. After 4 weeks, the animals were sacrificed, and specimens were collected for the inflammation test, including COX-2, iNOS, and nNOS in the injured skin and spinal cord dorsal horns through immunohistochemistry and Western assays. Furthermore, pro-inflammatory cytokines (IL-1 β and TNF-α) in the spinal cord were collected. Double immunofluorescent staining images for measuring p-IκB, p-NFκB, p-JNK, and TUNEL as well as Western blots of AKT, Bax/Bcl-2 for the inflammatory process, and apoptosis were analyzed. Fat grafting significantly reduced COX2, nNOS, and iNOS in the skin and spinal cord dorsal horns, as well as IL-1β and TNF-α, compared with the burn group. Moreover, regarding the anti-inflammatory effect, the apoptosis cells in the spinal cord significantly decreased after the fat grafting in the burn injury group. Fat grafting was effective in treating burn-induced neuropathic pain through the alleviation of neuroinflammation and ameliorated spinal neuronal apoptosis.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Burn injury
medicine.medical_treatment
lcsh:Medicine
Scars
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type I
Transplantation, Autologous
Rats, Sprague-Dawley
Cicatrix
medicine
Animals
lcsh:Science
Neuroinflammation
Inflammation
Multidisciplinary
business.industry
lcsh:R
medicine.disease
Spinal cord
Immunohistochemistry
Transplantation
medicine.anatomical_structure
Adipose Tissue
Spinal Cord
Cyclooxygenase 2
Anesthesia
Neuropathic pain
Tissue Transplantation
Neuralgia
Skin grafting
lcsh:Q
medicine.symptom
Nerve Net
business
Burns
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....8bf3896857e936bf9e19db69ab5c9f7e