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The complete 12 Mb genome and transcriptome of Nonomuraea gerenzanensis with new insights into its duplicated 'magic' RNA polymerase
- Source :
- Scientific Reports, Vol 6, Iss 1, Pp 1-13 (2016), Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Nature Portfolio, 2016.
-
Abstract
- In contrast to the widely accepted consensus of the existence of a single RNA polymerase in bacteria, several actinomycetes have been recently shown to possess two forms of RNA polymerases due the to co-existence of two rpoB paralogs in their genome. However, the biological significance of the rpoB duplication is obscure. In this study we have determined the genome sequence of the lipoglycopeptide antibiotic A40926 producer Nonomuraea gerenzanensis ATCC 39727, an actinomycete with a large genome and two rpoB genes, i.e. rpoB(S) (the wild-type gene) and rpoB(R) (the mutant-type gene). We next analyzed the transcriptional and metabolite profiles in the wild-type gene and in two derivative strains over-expressing either rpoB(R) or a mutated form of this gene to explore the physiological role and biotechnological potential of the “mutant-type” RNA polymerase. We show that rpoB(R) controls antibiotic production and a wide range of metabolic adaptive behaviors in response to environmental pH. This may give interesting perspectives also with regard to biotechnological applications.
- Subjects :
- 0301 basic medicine
Science
Genome
Article
03 medical and health sciences
chemistry.chemical_compound
Bacterial Proteins
RNA polymerase
Gene duplication
Actinomycetales
polycyclic compounds
Gene
Polymerase
Genetics
Whole genome sequencing
Multidisciplinary
biology
RNA
DNA-Directed RNA Polymerases
Hydrogen-Ion Concentration
biochemical phenomena, metabolism, and nutrition
rpoB
Anti-Bacterial Agents
030104 developmental biology
chemistry
Mutation
biology.protein
bacteria
Medicine
Teicoplanin
Transcriptome
Actinomycetes, Secondary metabolism, RNA polymerase, Rifampicin-resistance
Genome, Bacterial
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 6
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....8c36735216150ba11bf5bacc3a2a704c