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Combined effects of dietary fructooligosaccharide and Bacillus licheniformis on innate immunity, antioxidant capability and disease resistance of triangular bream (Megalobrama terminalis)
- Source :
- Fish & Shellfish Immunology. 35:1380-1386
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- This study was conducted to investigate the effects of fructooligosaccharide (FOS) and Bacillus licheniformis (B. licheniformis) and their interaction on innate immunity, antioxidant capability and disease resistance of triangular bream Megalobrama terminalis (average initial weight 30.5 ± 0.5 g). Nine experimental diets were formulated to contain three FOS levels (0, 0.3% and 0.6%) and three B. licheniformis levels (0, 1 × 10(7), 5 × 10(7) CFU g(-1)) according to a 3 × 3 factorial design. At the end of the 8-week feeding trial, fish were challenged by Aeromonas hydrophila (A. hydrophila) and survival rate was recorded for the next 7 days. The results showed that leucocyte counts, alternative complement activity as well as total serum protein and globulin contents all increased significantly (P0.05) as dietary B. licheniformis levels increased from 0 to 1 × 10(7) CFU g(-1), while little difference (P0.05) was observed in these parameters in terms of dietary FOS levels. Both plasma alkaline phosphatase and phenoloxidase activities were significantly (P0.05) affected only by dietary FOS levels with the highest values observed in fish fed 0.6 and 0.3% FOS, respectively. Both immunoglobulin M content and liver superoxide dismutase (SOD) activity were significantly affected (P0.05) by both FOS and B. licheniformis. Liver catalase, glutathione peroxidase as well as plasma SOD activities of fish fed 1 × 10(7) CFU g(-1)B. licheniformis were all significantly (P0.05) higher than that of the other groups, whereas the opposite was true for malondialdehyde content. After A. hydrophila challenge, survival rate was not affected (P0.05) by either FOS levels or B. licheniformis contents, whereas a significant (P0.05) interaction between these two substances was observed with the highest value observed in fish fed 0.3% FOS and 1 × 10(7) CFU g(-1)B. licheniformis. The results of this study indicated that dietary FOS and B. licheniformis could significantly enhance the innate immunity and antioxidant capability of triangular bream, as well as improve its disease resistance. The best combination of these two prebiotics and/or probiotics was 0.3% FOS and 1 × 10(7) CFU g(-1)B. licheniformis.
- Subjects :
- Antioxidant
Globulin
medicine.medical_treatment
Acid Phosphatase
Cyprinidae
Oligosaccharides
Bacillus
Aquaculture
Aquatic Science
Antioxidants
Microbiology
Dietary Carbohydrates
medicine
Animals
Environmental Chemistry
Food science
Bacillus licheniformis
Disease Resistance
chemistry.chemical_classification
Dose-Response Relationship, Drug
biology
Fructooligosaccharide
Glutathione peroxidase
Megalobrama terminalis
General Medicine
biology.organism_classification
Survival Analysis
Immunity, Innate
Aeromonas hydrophila
chemistry
Linear Models
biology.protein
Alkaline phosphatase
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....8c71f9ae51b9cef9a7d2e0f7407bfb7d