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Microsatellite Marker Discovery in the Stingless Bee Uruçu-Amarela (Melipona rufiventris Group, Hymenoptera, Meliponini) for Population Genetic Analysis
- Source :
- Insects, Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA, Volume 10, Issue 12
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- The species Melipona rufiventris Lepeletier, 1836 is a Brazilian native stingless bee that is part of a species complex known as the &lsquo<br />rufiventris group&rsquo<br />making it difficult to distinguish between the different species. Populations in this group are facing a severe decline, leading to the risk of local extinction, and therefore, their conservation should be treated as a major concern. This study describes the first set of tri- and tetranucleotide microsatellite markers, using next-generation sequencing technology for use in the identification of genetic diversity and population structure in the &lsquo<br />A total of 16 microsatellite loci displayed polymorphism. Analysis of the whole data set (n = 50) detected 63 alleles in all loci, ranging from 2 to 7 with a mean of 3.9 alleles/locus. A genetic diversity analysis revealed high values for population differentiation estimates (FST = 0.252, RST = 0.317, and DEST = 0.284) between the Atlantic Forest, Cerrado, and Caatinga biomes. An additional evidence for genetic divergence among populations was also found in the &rsquo<br />these should be treated as separate conservation units or even as separate species. These microsatellite markers have demonstrated a strong potential for assessing population discrimination in this threatened stingless bee group.
- Subjects :
- Species complex
molecular markers
Stingless bee
Population genetics
Population
Zoology
Biology
Genetic differentiation
Marcador Molecular
Article
Illumina platform
Genetic drift
Diferenciação genética
education
education.field_of_study
Genetic diversity
Conservação
conservation
population genetics
NGS technology
biology.organism_classification
Genetic divergence
Insect Science
Microsatellite
genetic differentiation
Genética populacional
Subjects
Details
- Language :
- English
- ISSN :
- 20754450
- Volume :
- 10
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Insects
- Accession number :
- edsair.doi.dedup.....8ca325c00fb8e2fbdf2ee81aa382f033