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High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet
- Source :
- Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group UK, 2017.
-
Abstract
- This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope. This non-scanning-type, high-resolution imaging method will be an effective tool for monitoring interfacial phenomena that exhibit relatively rapid reaction kinetics in various cellular and molecular dynamics.
- Subjects :
- 0301 basic medicine
Microscope
Materials science
Science
Nanoparticle
02 engineering and technology
Article
law.invention
03 medical and health sciences
Molecular dynamics
law
Fluorescence microscope
Surface plasmon resonance
Multidisciplinary
Total internal reflection fluorescence microscope
business.industry
021001 nanoscience & nanotechnology
Fluorescence
030104 developmental biology
Medicine
Optoelectronics
Nanometre
0210 nano-technology
business
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....8cae026923a3db6dfb55385c7c8152d6