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Anti-HIV-1 property of trichosanthin correlates with its ribosome inactivating activity
- Source :
- FEBS Letters. (2):295-298
- Publisher :
- Published by Elsevier B.V.
-
Abstract
- Trichosanthin (TCS) is a type I ribosome inactivating (RI) protein possessing anti-tumor and antiviral activity, including human immunodeficiency virus (HIV). The mechanism of these actions is not entirely clear, but is generally attributed to its RI property. In order to study the relationship between the anti-HIV-1 activity of TCS and its RI activity, three TCS mutants with different RI activities were constructed by using site-directed mutagenesis. The anti-HIV-1 activities of the three mutants were tested in vitro. Results showed that two TCS mutants, namely TCSM(120–123), TCSE160A/E189A, with the greatest decrease in RI activity, lost almost all of the anti-HIV activity and cytopathic effect. Another mutant TCSR122G, which exhibited a 160-fold decrease in RI activity, retained some anti-HIV activity. The results from this study suggested that RI activity of TCS may have significant contribution to its anti-HIV-1 property.
- Subjects :
- Trichosanthin
Anti-HIV Agents
Mutant
Biophysics
Mutagenesis (molecular biology technique)
Biology
medicine.disease_cause
Biochemistry
Cell Line
human immunodeficiency virus type 1
Structural Biology
Genetics
medicine
Animals
Amino Acid Sequence
Antiviral activity
Molecular Biology
Cytopathic effect
Mutation
Dose-Response Relationship, Drug
Ribosome-inactivating protein
fungi
Cell Biology
Ribosome inactivating protein
Molecular biology
In vitro
Cell culture
HIV-1
Rabbits
Ribosomes
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....8caf2d6abdcea7c83719b566019e03c4
- Full Text :
- https://doi.org/10.1016/S0014-5793(02)03539-1