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A fully automated microfluidic PCR-array system for rapid detection of multiple respiratory tract infection pathogens
- Source :
- Analytical and Bioanalytical Chemistry
- Publication Year :
- 2021
- Publisher :
- Springer Berlin Heidelberg, 2021.
-
Abstract
- Graphical abstract Rapid and accurate identification of respiratory tract infection pathogens is of utmost importance for clinical diagnosis and treatment, as well as prevention of pathogen transmission. To meet this demand, a microfluidic chip-based PCR-array system, Onestart, was developed. The Onestart system uses a microfluidic chip packaged with all the reagents required, and the waste liquid is also collected and stored on the chip. This ready-to-use system can complete the detection of 21 pathogens in a fully integrated manner, with sample lysis, nucleic acid extraction/purification, and real-time PCR sequentially implemented on the same chip. The entire analysis process is completed within 1.5 h, and the system automatically generates a test report. The lower limit-of-detection (LOD) of the Onestart assay was determined to be 1.0 × 103 copies·mL−1. The inter-batch variation of cycle threshold (Ct) values ranged from 0.08% to 0.69%, and the intra-batch variation ranged from 0.9% to 2.66%. Analytical results of the reference sample mix showed a 100% specificity of the Onestart assay. The analysis of batched clinical samples showed consistency of the Onestart assay with real-time PCR. With its ability to provide rapid, sensitive, and specific detection of respiratory tract infection pathogens, application of the Onestart system will facilitate timely clinical management of respiratory tract infections and effective prevention of pathogen transmission. Onestart, a ready-to-use system, can detect 21 pathogens in a fully integrated manner on a microchip within 1.5 h. Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03171-4.
- Subjects :
- Quality Control
Respiratory tract infection
Point-of-care testing
Microfluidics
Rapid diagnosis
02 engineering and technology
Biology
01 natural sciences
Biochemistry
Rapid detection
Polymerase Chain Reaction
Sensitivity and Specificity
Analytical Chemistry
Microbiology
law.invention
Pattern Recognition, Automated
Automation
COVID-19 Testing
law
Limit of Detection
Lab-On-A-Chip Devices
medicine
Humans
Diagnosis, Computer-Assisted
Pathogen
Respiratory Tract Infections
Polymerase chain reaction
Paper in Forefront
Detection limit
Respiratory tract infections
SARS-CoV-2
010401 analytical chemistry
Reproducibility of Results
Equipment Design
Microfluidic Analytical Techniques
021001 nanoscience & nanotechnology
0104 chemical sciences
medicine.anatomical_structure
Microfluidic
Viruses
RNA, Viral
0210 nano-technology
Respiratory tract
Multiplex detection
Subjects
Details
- Language :
- English
- ISSN :
- 16182650 and 16182642
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....8cba726ebd35571abca1f53f1e37ccc2