Supplementary Figures 1-5 and Supplmentary Materials and Methods. from Inhibition of Copper Transport Induces Apoptosis in Triple-Negative Breast Cancer Cells and Suppresses Tumor Angiogenesis

Figure S1 - Western blot images showing ATOX1 and CCS levels in TNBC cells, corresponding to data presented in Figure 1. Figure S2 - Dose-response profiles of TM in TNBC cells and DCAC50 in HMEC; caspase-3/7 activity after DCAC50 treatment of HMEC. Figure S3 - Copper content, copper distribution and iron and zinc content for DCAC50-treated TNBC cells, supplementing Figure 3. Figure S4 - Protein levels of copper transporters, GSH levels, GSH depletion experiments and SOD1 activity levels in TNBC cells in response to DCAC50 treatment, supplementing Figure 4. Figure S5 - Dose-response profile for DCAC50 and caspase-3/7 activity after DCAC50 treatment in HuVEC. Figure S6 - Evaluation of DCAC50 treatment on tumor volumes and angiogenesis in MDA-MB-468 and MDA-MB-231 xenograft mouse models, supplementing Figure 5. Supplementary Methods ¬- Information about the antibodies and detailed procedures used for Western blot, immunofluorescent staining and SOD activity assay.