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Long term stability of Oligo (dT) 25 magnetic beads for the expression analysis of Euglena gracilis for long term space projects
- Source :
- Life Sciences in Space Research. 13:12-18
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- The unicellular freshwater flagellate Euglena gracilis has a highly developed sensory system. The cells use different stimuli such as light and gravity to orient themselves in the surrounding medium to find areas for optimal growth. Due to the ability to produce oxygen and consume carbon dioxide, Euglena is a suitable candidate for life support systems. Participation in a long-term space experiment would allow for the analysis of changes and adaptations to the new environment, and this could bring new insights into the mechanism of perception of gravity and the associated signal transduction chain. For a molecular analysis of transcription patterns, an automated system is necessary, capable of performing all steps from taking a sample, processing it and generating data. One of the developmental steps is to find long-term stable reagents and materials and test them for stability at higher-than-recommended temperature conditions during extended storage time. We investigated the usability of magnetic beads in an Euglena specific lysis buffer after addition of the RNA stabilizer Dithiothreitol over 360 days and the lysis buffer with the stabilizer alone over 455 days at the expected storage temperature of 19 °C. We can claim that the stability is not impaired at all after an incubation period of over one year. This might be an interesting result for researchers who have to work under non-standard lab conditions, as in biological or medicinal fieldwork.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Euglena gracilis
RNA Stability
Health, Toxicology and Mutagenesis
ved/biology.organism_classification_rank.species
01 natural sciences
Euglena
Stability (probability)
Magnetics
03 medical and health sciences
Lysis buffer
Flagellate
Life support system
Magnetic beads
Radiation
Ecology
biology
ved/biology
Astronomy and Astrophysics
Space Flight
biology.organism_classification
Agricultural and Biological Sciences (miscellaneous)
Term (time)
Dithiothreitol
030104 developmental biology
Oligodeoxyribonucleotides
Biochemistry
Gravitationsbiologie
Biophysics
Stability
RNA, Protozoan
010606 plant biology & botany
Stabilizer (chemistry)
Subjects
Details
- ISSN :
- 22145524
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Life Sciences in Space Research
- Accession number :
- edsair.doi.dedup.....8d125773643f784b57fb6492e6c041db