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Emerging coexistence of three PMQR genes on a multiple resistance plasmid with a new surrounding genetic structure of qnrS2 in E. coli in China
- Source :
- Antimicrobial Resistance and Infection Control, Antimicrobial Resistance and Infection Control, Vol 9, Iss 1, Pp 1-8 (2020)
- Publication Year :
- 2019
-
Abstract
- Background Quinolones are commonly used for treatment of infections by bacteria of the Enterobacteriaceae family. However, the rising resistance to quinolones worldwide poses a major clinical and public health risk. This study aimed to characterise a novel multiple resistance plasmid carrying three plasmid-mediated quinolone resistance genes in Escherichia coli clinical stain RJ749. Methods MICs of ceftriaxone, cefepime, ceftazidime, ciprofloxacin, and levofloxacin for RJ749 and transconjugant c749 were determined by the Etest method. Conjugation was performed using sodium azide-resistant E. coli J53 strain as a recipient. The quinolone resistance-determining regions of gyrA, gyrB, parC, and parE were PCR-amplified. Results RJ749 was highly resistant to quinolones, while c749 showed low-level resistance. S1-nuclease pulsed-field gel electrophoresis revealed that RJ749 and c749 both harboured a plasmid. PCR presented chromosomal mutation sites of the quinolone resistance-determining region, which mediated quinolone resistance. The c749 genome comprised a single plasmid, pRJ749, with a multiple resistance region, including three plasmid-mediated quinolone resistance (PMQR) genes (aac (6′)-Ib-cr, qnrS2, and oqxAB) and ten acquired resistance genes. One of the genes, qnrS2, was shown for the first time to be flanked by two IS26s. Three IS26-mediated circular molecules carrying the PMQR genes were detected. Conclusions We revealed the coexistence of three PMQR genes on a multiple resistance plasmid and a new surrounding genetic structure of qnrS2 flanked by IS26 elements. IS26 plays an important role in horizontal spread of quinolone resistance.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
China
medicine.drug_class
Antibiotic resistance
030106 microbiology
Drug resistance
Levofloxacin
Microbial Sensitivity Tests
medicine.disease_cause
Ceftazidime
Plasmid
lcsh:Infectious and parasitic diseases
PMQR
03 medical and health sciences
qnrS2
Genome Size
Ciprofloxacin
Drug Resistance, Multiple, Bacterial
medicine
Escherichia coli
Pharmacology (medical)
lcsh:RC109-216
Cefepime
Gene
Genetics
biology
Whole Genome Sequencing
business.industry
Escherichia coli Proteins
Research
Ceftriaxone
Public Health, Environmental and Occupational Health
Horizontal gene transfer
biology.organism_classification
Quinolone
Enterobacteriaceae
Anti-Bacterial Agents
030104 developmental biology
Infectious Diseases
Mutation
business
Plasmids
Subjects
Details
- ISSN :
- 20472994
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Antimicrobial resistance and infection control
- Accession number :
- edsair.doi.dedup.....8d21cb9d3fb8f039f41960fade8dde1d