Back to Search
Start Over
Dietary silver nanoparticles can disturb the gut microbiota in mice
- Source :
- Particle and Fibre Toxicology, Particle and Fibre Toxicology, BioMed Central, 2016, 13, ⟨10.1186/s12989-016-0149-1⟩, Particle and Fibre Toxicology, Vol. 13, no. 1, p. 38 [1-16] (2016), Particle and Fibre Toxicology, 2016, 13, ⟨10.1186/s12989-016-0149-1⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- Background Humans are increasingly exposed via the diet to Ag nanoparticles (NP) used in the food industry. Because of their anti-bacterial activity, ingested Ag NP might disturb the gut microbiota that is essential for local and systemic homeostasis. We explored here the possible impact of dietary Ag NP on the gut microbiota in mice at doses relevant for currently estimated human intake. Methods Mice were orally exposed to food (pellets) supplemented with increasing doses of Ag NP (0, 46, 460 or 4600 ppb) during 28 d. Body weight, systemic inflammation and gut integrity were investigated to determine overall toxicity, and feces DNA collected from the gut were analyzed by Next Generation Sequencing (NGS) to assess the effect of Ag NP on the bacterial population. Ag NP were characterized alone and in the supplemented pellets by scanning transmission electron microscopy (STEM) and energy dispersive X-ray analysis (EDX). Results No overall toxicity was recorded in mice exposed to Ag NP. Ag NP disturbed bacterial evenness (α-diversity) and populations (β-diversity) in a dose-dependent manner. Ag NP increased the ratio between Firmicutes (F) and Bacteroidetes (B) phyla. At the family level, Lachnospiraceae and the S24-7 family mainly accounted for the increase in Firmicutes and decrease in Bacteroidetes, respectively. Similar effects were not observed in mice identically exposed to the same batch of Ag NP-supplemented pellets aged during 4 or 8 months and the F/B ratio was less or not modified. Analysis of Ag NP-supplemented pellets showed that freshly prepared pellets released Ag ions faster than aged pellets. STEM-EDX analysis also showed that Ag sulfidation occurred in aged Ag NP-supplemented pellets. Conclusions Our data indicate that oral exposure to human relevant doses of Ag NP can induce microbial alterations in the gut. The bacterial disturbances recorded after Ag NP are similar to those reported in metabolic and inflammatory diseases, such as obesity. It also highlights that Ag NP aging in food, and more specifically sulfidation, can reduce the effects of Ag NP on the microbiota by limiting the release of toxic Ag ions. Electronic supplementary material The online version of this article (doi:10.1186/s12989-016-0149-1) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Microscopy, Electron, Scanning Transmission
Silver
Firmicutes
Health, Toxicology and Mutagenesis
Metal Nanoparticles
[SDU.STU]Sciences of the Universe [physics]/Earth Sciences
010501 environmental sciences
Gut flora
Toxicology
01 natural sciences
Microbiology
03 medical and health sciences
Mice
medicine
Animals
Feces
0105 earth and related environmental sciences
Nanomaterials
2. Zero hunger
biology
Toxicity
Bacteria
Dose-Response Relationship, Drug
Research
Microbiota
Lachnospiraceae
Bacteroidetes
Spectrometry, X-Ray Emission
General Medicine
biology.organism_classification
medicine.disease
3. Good health
Intestines
030104 developmental biology
Food
NGS
Sulfidation
Dysbiosis
Subjects
Details
- Language :
- English
- ISSN :
- 17438977
- Database :
- OpenAIRE
- Journal :
- Particle and Fibre Toxicology, Particle and Fibre Toxicology, BioMed Central, 2016, 13, ⟨10.1186/s12989-016-0149-1⟩, Particle and Fibre Toxicology, Vol. 13, no. 1, p. 38 [1-16] (2016), Particle and Fibre Toxicology, 2016, 13, ⟨10.1186/s12989-016-0149-1⟩
- Accession number :
- edsair.doi.dedup.....8d4bff033e13b49a04238cf392db1fc0