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The ubiquitin specific protease USP34 protects the ubiquitin ligase gp78 from proteasomal degradation
- Source :
- Biochemical and biophysical research communications. 509(2)
- Publication Year :
- 2018
-
Abstract
- The E3 ubiquitin (Ub) ligase gp78 plays an important role in endoplasmic reticulum (ER)-associated degradation (ERAD) and regulation of lipid biogenesis. Although a variety of substrates of gp78 have been described, the regulation of the degradation of gp78 itself remains poorly understood. To address this problem, we used co-immunoprecipitation-coupled liquid chromatography-tandem mass spectrometry (Co-IP/LC-MS/MS) to identify novel proteins interacting with gp78. One of the proteins identified in this study is the deubiquitylating (DUB) enzyme USP34 (Ub-specific protease 34). We demonstrate that knockdown of USP34 facilitates proteasomal degradation of gp78 and consequently impairs the function of gp78 in regulating lipid droplet formation. This study unveils a previously unknown function of USP34 in regulating the metabolic stability of gp78 and adds to our understanding of the relevance of partnering of DUBs and E3s in regulation of protein ubiquitylation.
- Subjects :
- 0301 basic medicine
Proteasome Endopeptidase Complex
Biophysics
Protein degradation
Endoplasmic-reticulum-associated protein degradation
Endoplasmic Reticulum
Biochemistry
03 medical and health sciences
0302 clinical medicine
Ubiquitin
Lipid droplet
Cell Line, Tumor
Humans
RNA, Small Interfering
Molecular Biology
chemistry.chemical_classification
DNA ligase
biology
Chemistry
Endoplasmic reticulum
Ubiquitination
Cell Biology
Endoplasmic Reticulum-Associated Degradation
Lipid Droplets
Lipid Metabolism
Ubiquitin ligase
Cell biology
Receptors, Autocrine Motility Factor
030104 developmental biology
HEK293 Cells
030220 oncology & carcinogenesis
Proteolysis
biology.protein
Hepatocytes
Ubiquitin-Specific Proteases
Biogenesis
Protein Binding
Signal Transduction
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 509
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....8d5b34d56152dfb5b37f9d89df1c7045