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Deep RNA sequencing of pectoralis muscle transcriptomes during late-term embryonic to neonatal development in indigenous Chinese duck breeds
- Source :
- PLoS ONE, PLoS ONE, Vol 12, Iss 8, p e0180403 (2017)
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Pectoral muscle (PM) comprises an important component of overall meat mass in ducks. However, PM has shown arrested or even reduced growth during late embryonic development, and the molecular mechanisms underlying PM growth during the late embryonic to neonatal period in ducks have not been addressed. In this study, we characterized potential candidate genes and signaling pathways related to PM development using RNA sequencing of PM samples selected at embryonic days (E) 21 and 27 and 5 days post-hatch (dph) in two duck breeds (Gaoyou and Jinding ducks). A total of 393 differentially expressed genes (DEGs) were identified, which showed higher or lower expression levels at E27 compared with E21 and 5 dph, reflecting the pattern of PM growth rates. Among these, 43 DEGs were common to all three time points in both duck breeds. These DEGs may thus be involved in regulating this developmental process. Specifically, KEGG pathway analysis of the 393 DEGs showed that genes involved with different metabolism pathways were highly expressed, while genes involved with cell cycle pathways showed lower expression levels at E27. These DEGs may thus be involved in the mechanisms responsible for the phenomenon of static or decreased breast muscle growth in duck breeds during the late embryonic period. These results increase the available genetic information for ducks and provide valuable resources for analyzing the mechanisms underlying the process of PM development.
- Subjects :
- Male
0301 basic medicine
Molecular biology
Waterfowl
lcsh:Medicine
Gene Expression
Biochemistry
Poultry
Transcriptome
Sequencing techniques
Medicine and Health Sciences
Morphogenesis
lcsh:Science
Musculoskeletal System
Regulation of gene expression
Genetics
Multidisciplinary
Muscles
Gene Ontologies
Gene Expression Regulation, Developmental
High-Throughput Nucleotide Sequencing
RNA sequencing
Genomics
Muscle Differentiation
Ducks
Vertebrates
Female
Metabolic Pathways
Anatomy
Research Article
Sequence analysis
Period (gene)
Motor Proteins
Embryonic Development
Biology
Pectoralis Muscles
Birds
03 medical and health sciences
Molecular Motors
Animals
Gene
Sequence Analysis, RNA
Gene Expression Profiling
lcsh:R
Embryogenesis
Organisms
Biology and Life Sciences
Proteins
Computational Biology
RNA
Cell Biology
Genome Analysis
Research and analysis methods
Gene expression profiling
Molecular biology techniques
Metabolism
030104 developmental biology
Skeletal Muscles
Fowl
Amniotes
lcsh:Q
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....8d766e510d2669b725af2cb74e9b0997