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Semen phthalate metabolites, spermatozoa apoptosis, and DNA damage: a cross-sectional study in China
- Source :
- Environmental sciencetechnology. 49(6)
- Publication Year :
- 2015
-
Abstract
- Toxicological studies have shown that phthalates, a class of widely used chemicals, can impair male reproductive function, but epidemiological evidence is inconsistent. This study aimed to investigate the associations of semen phthalate metabolites with sperm apoptosis and DNA damage in a Chinese population. We assessed sperm apoptosis markers with Annexin V/PI analysis and sperm DNA integrity with comet assay before measuring eight phthalate metabolites in semen by high-performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS) among 463 men from Wuhan, China. We found a suggestive dose-response relationship between semen mono-(2-ethylhexyl) phthalate (MEHP) and an increased percentage of Annexin V+/PI- sperm (p for trend of0.10). We also observed that semen monomethyl phthalate (MMP) and monoethyl phthalate (MEP) were associated with significant dose-related increases in tail length of the comet (both p for trend of0.01). In conclusion, our data indicate that semen MEHP is associated with increased sperm apoptosis and that semen MMP and MEP are associated with increased sperm DNA damage in a Chinese population.
- Subjects :
- Adult
Male
endocrine system
China
DNA damage
Phthalic Acids
Semen
Apoptosis
Tandem mass spectrometry
Andrology
chemistry.chemical_compound
Annexin
Environmental Chemistry
Humans
urogenital system
Phthalate
General Chemistry
Sperm
Spermatozoa
Comet assay
Cross-Sectional Studies
chemistry
Regression Analysis
Comet Assay
DNA Damage
Subjects
Details
- ISSN :
- 15205851
- Volume :
- 49
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Environmental sciencetechnology
- Accession number :
- edsair.doi.dedup.....8db3ab8172d3541bd3f26ab0f0edd05a