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Development of novel dental restorative composites with dibasic calcium phosphate loaded chitosan fillers
- Source :
- Dental materials : official publication of the Academy of Dental Materials. 36(4)
- Publication Year :
- 2019
-
Abstract
- The incorporation of antimicrobial agents in restorative dental composites has the potential to slow the development of carious lesions.The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.Chitosan and chitosan/DCPA particles were synthetized by the electrospray method. Experimental composites were formulated by adding 0, 0.5, or 1.0 wt% particles into a resin matrix along with 60 wt% barium glass. The degree of conversion and mechanical properties were measured after 1 and 90 days of aging in water after photoactivation. Cytotoxicity and genotoxicity were evaluated using fibroblasts from dental pulp in conditioned medium. The antimicrobial activity against Streptococcus mutans was assessed by crystal violet biofilm assay.The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively. The antimicrobial results showed that all composites with approximately 20% less biofilm (p0.001) relative to the control. No chitosan release was detected from the composites, suggesting direct contact of the bacteria with exposed chitosan particles on the surface was responsible for the observed antimicrobial effect. The addition of the chitosan and chitosan/DCPA submicrometer (250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.It can be concluded that the addition of chitosan or chitosan/DCPA particles in the restorative composites induced antimicrobial activity without compromising the mechanical properties or biocompatibility of the composites.
- Subjects :
- Calcium Phosphates
Materials science
Biocompatibility
Surface Properties
02 engineering and technology
medicine.disease_cause
Composite Resins
Chitosan
Streptococcus mutans
03 medical and health sciences
chemistry.chemical_compound
Dental Materials
0302 clinical medicine
Flexural Strength
Materials Testing
medicine
General Materials Science
Crystal violet
Composite material
Pliability
General Dentistry
biology
Dibasic acid
Biomaterial
030206 dentistry
021001 nanoscience & nanotechnology
biology.organism_classification
Antimicrobial
chemistry
Mechanics of Materials
0210 nano-technology
Genotoxicity
Subjects
Details
- ISSN :
- 18790097
- Volume :
- 36
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Dental materials : official publication of the Academy of Dental Materials
- Accession number :
- edsair.doi.dedup.....8dcd0768a53bc010b63697916ccd460a