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Differential Expression and Pathway Analysis in Drug-Resistant Triple-Negative Breast Cancer Cell Lines Using RNASeq Analysis
- Source :
- International Journal of Molecular Sciences, Volume 19, Issue 6, International Journal of Molecular Sciences, Vol 19, Iss 6, p 1810 (2018)
- Publication Year :
- 2018
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2018.
-
Abstract
- Triple-negative breast cancer (TNBC) is among the most notorious types of breast cancer, the treatment of which does not give consistent results due to the absence of the three receptors (estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) as well as high amount of molecular variability. Drug resistance also contributes to treatment unresponsiveness. We studied differentially expressed genes, their biological roles, as well as pathways from RNA-Seq datasets of two different TNBC drug-resistant cell lines of Basal B subtype SUM159 and MDA-MB-231 treated with drugs JQ1 and Dexamethasone, respectively, to elucidate the mechanism of drug resistance. RNA sequencing(RNA-Seq) data analysis was done using edgeR which is an efficient program for determining the most significant Differentially Expressed Genes (DEGs), Gene Ontology (GO) terms, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. iPathway analysis was further used to obtain validated results using analysis that takes into consideration type, function, and interactions of genes in the pathway. The significant similarities and differences throw light into the molecular heterogeneity of TNBC, giving clues into the aspects that can be focused to overcome drug resistance. From this study, cytokine-cytokine receptor interaction pathway appeared to be a key factor in TNBC drug resistance.
- Subjects :
- 0301 basic medicine
Estrogen receptor
Antineoplastic Agents
Triple Negative Breast Neoplasms
Drug resistance
Biology
RNASeq
Dexamethasone
Article
Catalysis
lcsh:Chemistry
Inorganic Chemistry
03 medical and health sciences
0302 clinical medicine
Breast cancer
Triple-negative breast cancer
Cell Line, Tumor
Progesterone receptor
medicine
Humans
Receptors, Cytokine
Physical and Theoretical Chemistry
KEGG
Receptor
lcsh:QH301-705.5
Molecular Biology
Gene
Spectroscopy
cytokine-cytokine receptor interaction
drug resistance
basal b
Organic Chemistry
Azepines
General Medicine
Triazoles
medicine.disease
Computer Science Applications
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Drug Resistance, Neoplasm
030220 oncology & carcinogenesis
Cancer research
Cytokines
Transcriptome
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....8dd241badaebd9531516d122db40391e
- Full Text :
- https://doi.org/10.3390/ijms19061810