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Fluorescence Correlation Spectroscopy Monitors the Fate of Degradable Nanocarriers in the Blood Stream

Authors :
Sascha Schmitt
Anne Huppertsberg
Adrian Klefenz
Leonard Kaps
Volker Mailänder
Detlef Schuppan
Hans-Jürgen Butt
Lutz Nuhn
Kaloian Koynov
Source :
Biomacromolecules
Publication Year :
2022
Publisher :
American Chemical Society (ACS), 2022.

Abstract

The use of nanoparticles as carriers to deliver pharmacologically active compounds to specific parts of the body via the bloodstream is a promising therapeutic approach for the effective treatment of various diseases. To reach their target sites, nanocarriers (NCs) need to circulate in the bloodstream for prolonged periods without aggregation, degradation, or cargo loss. However, it is very difficult to identify and monitor small-sized NCs and their cargo in the dense and highly complex blood environment. Here, we present a new fluorescence correlation spectroscopy-based method that allows the precise characterization of fluorescently labeled NCs in samples of less than 50 μL of whole blood. The NC size, concentration, and loading efficiency can be measured to evaluate circulation times, stability, or premature drug release. We apply the new method to follow the fate of pH-degradable fluorescent cargo-loaded nanogels in the blood of live mice for periods of up to 72 h.

Details

ISSN :
15264602 and 15257797
Volume :
23
Database :
OpenAIRE
Journal :
Biomacromolecules
Accession number :
edsair.doi.dedup.....8e110ca5bf0bdc48cc5f2f0c2a3d9a44
Full Text :
https://doi.org/10.1021/acs.biomac.1c01407