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Systematic identification and characterization of novel human skin-associated genes encoding membrane and secreted proteins
- Source :
- PLoS ONE, Vol 8, Iss 6, p e63949 (2013), PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Through bioinformatics analyses of a human gene expression database representing 105 different tissues and cell types, we identified 687 skin-associated genes that are selectively and highly expressed in human skin. Over 50 of these represent uncharacterized genes not previously associated with skin and include a subset that encode novel secreted and plasma membrane proteins. The high levels of skin-associated expression for eight of these novel therapeutic target genes were confirmed by semi-quantitative real time PCR, western blot and immunohistochemical analyses of normal skin and skin-derived cell lines. Four of these are expressed specifically by epidermal keratinocytes; two that encode G-protein-coupled receptors (GPR87 and GPR115), and two that encode secreted proteins (WFDC5 and SERPINB7). Further analyses using cytokine-activated and terminally differentiated human primary keratinocytes or a panel of common inflammatory, autoimmune or malignant skin diseases revealed distinct patterns of regulation as well as disease associations that point to important roles in cutaneous homeostasis and disease. Some of these novel uncharacterized skin genes may represent potential biomarkers or drug targets for the development of future diagnostics or therapeutics.
- Subjects :
- Keratinocytes
Male
Skin Neoplasms
lcsh:Medicine
Human skin
Atopic Dermatitis
Biochemistry
Transcriptome
0302 clinical medicine
Gene expression
Pathology
Receptors, Lysophosphatidic Acid
lcsh:Science
Cells, Cultured
Skin
0303 health sciences
Multidisciplinary
medicine.diagnostic_test
integumentary system
Genomics
Milk Proteins
Clinical Laboratory Sciences
3. Good health
Cell biology
Functional Genomics
Organ Specificity
030220 oncology & carcinogenesis
Cytochemistry
Medicine
Female
Research Article
Skin Infections
Cell type
Inflammatory Diseases
Primary Cell Culture
Dermatology
Biology
Skin Diseases
03 medical and health sciences
Western blot
Diagnostic Medicine
medicine
Humans
Gene
Serpins
030304 developmental biology
Cell Membrane
lcsh:R
Membrane Proteins
Molecular Sequence Annotation
Molecular biology
Secretory protein
Membrane protein
lcsh:Q
Genome Expression Analysis
Biomarkers
Interleukin-1
General Pathology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....8e2b54e45262516e4acaca991a62cfe6