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Activin B Promotes Epithelial Wound Healing In Vivo through RhoA-JNK Signaling Pathway
- Source :
- PLoS ONE, PLoS ONE, Vol 6, Iss 9, p e25143 (2011)
- Publication Year :
- 2011
- Publisher :
- Public Library of Science (PLoS), 2011.
-
Abstract
- Background Activin B has been reported to promote the proliferation and migration of keratinocytes in vitro via the RhoA-JNK signaling pathway, whereas its in vivo role and mechanism in wound healing process has not yet been elucidated. Principal Findings In this study, we explored the potential mechanism by which activin B induces epithelial wound healing in mice. Recombinant lentiviral plasmids, with RhoA (N19) and RhoA (L63) were used to infect wounded KM mice. The wound healing process was monitored after different treatments. Activin B-induced cell proliferation on the wounded skin was visualized by electron microscopy and analyzed by 5′-bromodeoxyuridine (BrdU) incorporation assay. Protein expression of p-JNK or p-cJun was determined by immunohistochemical staining and immunoblotting analysis. Activin B efficiently stimulated the proliferation of keratinocytes and hair follicle cells at the wound area and promoted wound closure. RhoA positively regulated activin B-induced wound healing by up-regulating the expression of p-JNK and p-cJun. Moreover, suppression of RhoA activation delayed activin B-induced wound healing, while JNK inhibition recapitulated phenotypes of RhoA inhibition on wound healing. Conclusion These results demonstrate that activin B promotes epithelial wound closure in vivo through the RhoA-Rock-JNK-cJun signaling pathway, providing novel insight into the essential role of activin B in the therapy of wound repair.
- Subjects :
- Keratinocytes
Male
RHOA
MAP Kinase Signaling System
Blotting, Western
lcsh:Medicine
Apoptosis
Signaling Pathways
Immunoenzyme Techniques
Mice
Cell Movement
Molecular Cell Biology
medicine
Animals
Phosphorylation
lcsh:Science
Biology
Cells, Cultured
Activin type 2 receptors
Cell Proliferation
Wound Healing
rho-Associated Kinases
Multidisciplinary
integumentary system
biology
Cell growth
lcsh:R
Lentivirus
JNK Mitogen-Activated Protein Kinases
Epithelial Cells
Cell migration
Hair follicle
Activins
Cell biology
medicine.anatomical_structure
biology.protein
lcsh:Q
Female
Cellular Types
Signal transduction
rhoA GTP-Binding Protein
Wound healing
Hair Follicle
Research Article
Signal Transduction
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....8e55552c40402f22eec3091295a2f63a