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Illumina MiSeq sequencing and network analysis the distribution and co-occurrence of bacterioplankton in Danjiangkou Reservoir, China
- Source :
- Archives of Microbiology. 202:859-873
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Network analysis has contributed to studies of the interactions of microorganisms and the identification of key populations. However, such analysis has rarely been conducted in the study of reservoir bacterioplankton communities. This study investigated the bacterioplankton community composition in the surface water of the Danjiangkou Reservoir using the Illumina MiSeq sequencing platform. We observed that the bacterioplankton community primarily consisted of 27 phyla and 336 genera, including Actinobacteria, Proteobacteria, and Bacteroidetes, demonstrating the richness of the community composition. Redundancy analysis of the bacterioplankton communities and environmental variables showed that the total nitrogen (TN), pH, chemical oxygen demand (COD), and permanganate index (CODMn) were important factors affecting the bacterioplankton distribution. Network analysis was performed using the relative abundances of bacterioplankton based on the phylogenetic molecular ecological network (pMEN) method. The connectivity of node i within modules (Zi), the connectivity of node i among modules (Pi), and the number of key bacteria were high at the Taizishan and Heijizui sites, which were associated with higher TN contents than at the other sites. Among the physicochemical properties of water, TN, ammonia nitrogen (NH4-N), pH, COD, and dissolved oxygen (DO) might have great influences on the functional units of the bacterial communities in bacterioplankton molecular networks. This study improves the understanding of the structure and function of bacterioplankton communities in the Danjiangkou Reservoir.
- Subjects :
- Aquatic Organisms
China
Microorganism
Fresh Water
Biochemistry
Microbiology
Actinobacteria
03 medical and health sciences
Microbial ecology
Genetics
Molecular Biology
Phylogeny
Demography
030304 developmental biology
0303 health sciences
Bacteria
biology
030306 microbiology
Ecology
Chemical oxygen demand
High-Throughput Nucleotide Sequencing
Bacteroidetes
Biodiversity
General Medicine
Bacterioplankton
Plankton
biology.organism_classification
Species richness
Proteobacteria
Subjects
Details
- ISSN :
- 1432072X and 03028933
- Volume :
- 202
- Database :
- OpenAIRE
- Journal :
- Archives of Microbiology
- Accession number :
- edsair.doi.dedup.....8e59d39e876cc7ea6124c3a8aefdc800