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miR-330 Regulates Colorectal Cancer Oncogenesis by Targeting BACH1
- Source :
- Advanced Pharmaceutical Bulletin, Vol 10, Iss 3, Pp 444-451 (2020), Advanced Pharmaceutical Bulletin, Shirjang, S, Mansoori, B, Mohammad, A, Shajari, N, Duijf, P H G, Najaf, S, Gaballu, F A, Nofouzi, K & Baradaran, B 2020, ' miR-330 Regulates Colorectal Cancer Oncogenesis by Targeting BACH1 ', Advanced pharmaceutical bulletin, vol. 10, no. 3, pp. 444-451 . https://doi.org/10.34172/apb.2020.054
- Publication Year :
- 2020
- Publisher :
- Tabriz University of Medical Sciences, 2020.
-
Abstract
- Purpose: Based on WHO report, colorectal cancer (CRC) is the second cause of death among patients with cancer worldwide. Dysregulation of miRNAs expressions has been demonstrated in different human cancers, especially CRC. Studies have shown that miR-330 could act as both TS-miR and/or oncomiR in different types of cancers. BACH1 is also identified as a transcription factor, which is involved in ontogenesis. In this study, we evaluated the CRC suppression via silencing of BACH1 by small silencer molecule called miR-330.Methods:Firstly, we analyzed the BACH1, miR-330-3p and miR-330-5p expressions according to the colon adenocarcinoma (COAD) and rectal adenocarcinoma (READ) project established from a patient of the colon and rectal cancer patients in The Cancer Genome Atlas (TCGA) database. The targeting of BACH1 via miR-330 in human CRC cells was evaluated by Vejnar bioinformatics methods, and confirmed by qRT-PCR and western blot analysis. Proliferation was performed by MTT assay. The MMP9, CXCR4, and VEGFR proteins were measured by western blotting.Results:The analysis of BACH1, miR-330-3p, and miR-330-5p expressions according to the COAD and READ projects showed that BACH1 was overexpressed, but miR-330-3p and miR330-5p were reduced in CRC tumors compared to normal controls. The miR-330 induction prevented proliferation of CRC cell by targeting BACH1 mRNA, which represses MMP9, C-X-C chemokine receptor type 4 (CXCR4), and vascular endothelial growth factor receptor (VEGFR) proteins expressions.Conclusion:Our results suggested that BACH1 is a potential target for miR-330 in CRC cells. The miR-330 induction inhibits CRC cells proliferation by suppressing BACH1 expression in posttranscriptional level. It was suggested that targeting of BACH1 via miRNA such as miR-330 could be a valid strategy in the field of CRC targeted therapy via modulating the oncogenic signaling pathway.
- Subjects :
- Colorectal cancer
medicine.medical_treatment
proliferation
Pharmaceutical Science
colorectal cancer
BACH1
medicine.disease_cause
CXCR4
miR-330
Targeted therapy
VEGFR
03 medical and health sciences
mir-330
0302 clinical medicine
microRNA
medicine
Gene silencing
General Pharmacology, Toxicology and Pharmaceutics
030304 developmental biology
0303 health sciences
MMP9
business.industry
bach1
lcsh:RM1-950
vegfr
Oncomir
medicine.disease
digestive system diseases
Blot
lcsh:Therapeutics. Pharmacology
030220 oncology & carcinogenesis
Cancer research
mmp9
Carcinogenesis
business
cxcr4
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 22517308 and 22285881
- Volume :
- 10
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Advanced Pharmaceutical Bulletin
- Accession number :
- edsair.doi.dedup.....8e6fba93f8a278a61e95e484c6d2e0b7