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Junctional sequences influence the specificity of gamma/delta T cell receptors
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 1991
- Publisher :
- Rockefeller University Press, 1991.
-
Abstract
- T lymphocytes bearing the gamma/delta T cell receptor (TCR-gamma/delta) express a limited number of germline variable gene segments, generating receptor sequence diversity primarily through junctional mechanisms. To examine the role of V(D)J junctional sequences in antigen recognition by TCR-gamma/delta, we derived an alloreactive murine TCR-gamma/delta+ T cell line, LKD1, specific for the I-Ad class II major histocompatibility complex (MHC) molecule, and compared its receptor with that expressed by a previously characterized class II MHC alloreactive T cell line, LBK5, specific for I-Ek,b,s Ia molecules. Both LKD1 and LBK5 express receptors encoded by rearranged V gamma 1.2J gamma 2 and V delta 5D delta 2J delta 1 gene elements, differing in sequence only in the V(D)J junctional regions of the gamma and delta genes. These results demonstrate that junctionally encoded sequences corresponding to the putative third complementarity determining region can influence the antigen specificity of TCR-gamma/delta.
- Subjects :
- Cytotoxicity, Immunologic
T-Lymphocytes
T cell
Molecular Sequence Data
Immunology
Receptors, Antigen, T-Cell
Mice, Inbred Strains
Complementarity determining region
Biology
Major histocompatibility complex
Cell Line
Epitopes
Mice
Antigen
medicine
Animals
Immunology and Allergy
Receptor
Gamma delta T cell
Genetics
Base Sequence
T-cell receptor
Histocompatibility Antigens Class II
Antibodies, Monoclonal
Receptors, Antigen, T-Cell, gamma-delta
Articles
DNA
Gene rearrangement
Molecular biology
medicine.anatomical_structure
biology.protein
Subjects
Details
- ISSN :
- 15409538 and 00221007
- Volume :
- 173
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....8e72aedd9490a316e008906a23f40b40