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Expression of 8-OHdG in Zosterisessor ophiocephalus from the Venetian lagoon, Italy
- Source :
- European Journal of Histochemistry, Vol 57, Iss 1, Pp e8-e8 (2013), Scopus-Elsevier, European Journal of Histochemistry : EJH
- Publication Year :
- 2013
-
Abstract
- The aim of the present work was to evaluate the expression of 8-OHdG (8-hydroxydeoxyguanosine) in the benthic fish Zosterisessor ophiocephalus collected in two differently polluted sites of the Venetian lagoon (Porto Marghera and Caroman). We compared our data on 8-OHdG with those of CYP1A (Cytochrome P450, family 1, subfamily A, polypeptide 1), which is a well known biomarker for detoxification of contaminants. Immunohistochemistry with an antibody to 8-OHdG showed immunopositivity in nuclei of hepatocytes as well as in melanomacrophage centres of spleen and kidney, whereas an anti-CYP1A antibody exhibited positive immunostaining in the liver, kidney and ovary. The liver of males showed higher expression of both proteins than females. In animals from Porto Marghera site, the enzymatic assay for 8-OHdG exhibited higher levels in liver of males than in females. Western Blot analysis using the antibody anti-CYP1A recognized the presence of a band of about 60 kDa in the liver of males and females. Males exhibited a strong band, whereas in females the band showed a lower intensity. By using Real-Time PCR, the mRNA expression of CYP1A did not show any differences between males and females from each site, but it was at borderline significance level. Comparing the two sites, mRNA expression of CYP1A was significantly higher in the liver of both males and females from Porto Marghera than that of Caroman. The present data suggest that pollutants are bio-available as demonstrated by our biomarker analyses and may have a harmful effect on aquatic organisms such as Z. ophiocephalus. We report that the highest levels of hepatic 8-OHdG and CYP1A expression were detected in males, showing clear gender specificity.
- Subjects :
- Fish Proteins
Male
Pathology
medicine.medical_specialty
Histology
Biophysics
molecular biology
Ovary
Perciformes
Gene Expression Regulation, Enzymologic
Andrology
Western blot
medicine
Cytochrome P-450 CYP1A1
Animals
Water Pollutants
lcsh:QH301-705.5
Cell Nucleus
fish
Original Paper
Sex Characteristics
biology
medicine.diagnostic_test
CYP1A
Cytochrome P450
Deoxyguanosine
Cell Biology
biology.organism_classification
medicine.anatomical_structure
OHdG
Italy
lcsh:Biology (General)
8-Hydroxy-2'-Deoxyguanosine
Organ Specificity
immunohistochemistry
biology.protein
Biomarker (medicine)
Immunohistochemistry
Female
Antibody
Immunostaining
8-OHdG
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- European Journal of Histochemistry, Vol 57, Iss 1, Pp e8-e8 (2013), Scopus-Elsevier, European Journal of Histochemistry : EJH
- Accession number :
- edsair.doi.dedup.....8e75900f68f7ee3441a40b42f18db406