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Antioxidant, antimicrobial, antiparasitic, and cytotoxic properties of various Brazilian propolis extracts
- Source :
- PLoS ONE, Vol 12, Iss 3, p e0172585 (2017), PLoS ONE
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Propolis is known for its biological properties and its preparations have been continuously investigated in an attempt to solve the problem of their standardization, an issue that limits the use of propolis in food and pharmaceutical industries. The aim of this study was to evaluate in vitro antioxidant, antimicrobial, antiparasitic, and cytotoxic effects of extracts of red, green, and brown propolis from different regions of Brazil, obtained by ethanolic and supercritical extraction methods. We found that propolis extracts obtained by both these methods showed concentration-dependent antioxidant activity. The extracts obtained by ethanolic extraction showed higher antioxidant activity than that shown by the extracts obtained by supercritical extraction. Ethanolic extracts of red propolis exhibited up to 98% of the maximum antioxidant activity at the highest extract concentration. Red propolis extracts obtained by ethanolic and supercritical methods showed the highest levels of antimicrobial activity against several bacteria. Most extracts demonstrated antimicrobial activity against Staphylococcus aureus. None of the extracts analyzed showed activity against Escherichia coli or Candida albicans. An inhibitory effect of all tested ethanolic extracts on the growth of Trypanosoma cruzi Y strain epimastigotes was observed in the first 24 h. However, after 96 h, a persistent inhibitory effect was detected only for red propolis samples. Only ethanolic extracts of red propolis samples R01Et.B2 and R02Et.B2 showed a cytotoxic effect against all four cancer cell lines tested (HL-60, HCT-116, OVCAR-8, and SF-295), indicating that red propolis extracts have great cytotoxic potential. The biological effects of ethanolic extracts of red propolis revealed in the present study suggest that red propolis can be a potential alternative therapeutic treatment against Chagas disease and some types of cancer, although high activity of red propolis in vitro needs to be confirmed by future in vivo investigations.
- Subjects :
- 0301 basic medicine
Life Cycles
Antioxidant
Staphylococcus
medicine.medical_treatment
lcsh:Medicine
Protozoology
Pathology and Laboratory Medicine
medicine.disease_cause
Biochemistry
Antioxidants
Geographical locations
Anti-Infective Agents
Candida albicans
Medicine and Health Sciences
Staphylococcus Aureus
lcsh:Science
Chromatography, High Pressure Liquid
Extraction Techniques
Protozoans
Trypanosoma Cruzi
Multidisciplinary
Antiparasitic Agents
biology
Traditional medicine
Antimicrobials
Organic Compounds
Chemistry
Supercritical fluid extraction
Drugs
04 agricultural and veterinary sciences
Antimicrobial
040401 food science
Bacterial Pathogens
Medical Microbiology
Staphylococcus aureus
Epimastigotes
Physical Sciences
Protozoan Life Cycles
Pathogens
Brazil
Research Article
Trypanosoma
Antiparasitic
medicine.drug_class
Microbial Sensitivity Tests
Research and Analysis Methods
Microbiology
Propolis
03 medical and health sciences
0404 agricultural biotechnology
In vivo
Microbial Control
Escherichia coli
medicine
Humans
Supercritical Fluid Extraction
Microbial Pathogens
Cell Proliferation
Pharmacology
Bacteria
Ethanol
Organic Chemistry
lcsh:R
Organisms
Chemical Compounds
Biology and Life Sciences
South America
biology.organism_classification
Parasitic Protozoans
030104 developmental biology
Alcohols
lcsh:Q
People and places
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....8e7d4698347d1471298a3c84a0557adf
- Full Text :
- https://doi.org/10.1371/journal.pone.0172585