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Effects of extracellular acid stimulation on rat vascular smooth muscle cell in Gas6/Axl or PI3K/Akt signaling pathway
- Source :
- Clinical and experimental hypertension (New York, N.Y. : 1993). 38(5)
- Publication Year :
- 2016
-
Abstract
- Recent studies have indicated that extracellular acid stimulation inhibited the calcification of vascular smooth muscle cells (VSMCs). Cell apoptosis played an important role in the occurrence and development of vascular calcification. We further explored the effects of Gas6/Axl or PI3K/Akt signaling pathway on the inhibition of rat VSMCs calcification in response to extracellular acid stimulation. Our study demonstrated that a high concentration of phosphorus induced apoptosis and calcification of VSMCs, decreased expression of Axl, and reduced phosphorylation of Akt. Stimulation of extracellular acid counteracted the effects as above by increasing the expression of Axl and Akt phosphorylation and decreasing the expression of activated Caspase3, which thereby decreased cell apoptosis and calcification. Moreover, the effects can be attenuated by PI3K inhibitor. Our study proved that extracellular acid stimulation played a vital role in the inhibition of rat VSMCs calcification and apoptosis in Gas6/Axl or PI3K/Akt signaling pathway.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Vascular smooth muscle
Physiology
Apoptosis
030204 cardiovascular system & hematology
Biology
Muscle, Smooth, Vascular
Rats, Sprague-Dawley
03 medical and health sciences
Phosphatidylinositol 3-Kinases
0302 clinical medicine
Internal medicine
Internal Medicine
medicine
Extracellular
Animals
Phosphorylation
Protein kinase B
Protein Kinase Inhibitors
PI3K/AKT/mTOR pathway
Phosphoinositide-3 Kinase Inhibitors
Akt/PKB signaling pathway
GAS6
Caspase 3
Calcinosis
Receptor Protein-Tyrosine Kinases
General Medicine
medicine.disease
Cell biology
030104 developmental biology
Endocrinology
Hypertension
Quinolines
Intercellular Signaling Peptides and Proteins
Indicators and Reagents
Signal transduction
Proto-Oncogene Proteins c-akt
Calcification
Signal Transduction
Subjects
Details
- ISSN :
- 15256006
- Volume :
- 38
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Clinical and experimental hypertension (New York, N.Y. : 1993)
- Accession number :
- edsair.doi.dedup.....8e849b0c8c3c4689a0d1069bba579a4b