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Ethylene promotes pollen tube growth by affecting actin filament organization via the cGMP-dependent pathway in Arabidopsis thaliana
- Source :
- Protoplasma. 255(1)
- Publication Year :
- 2017
-
Abstract
- Ethylene and cGMP are key regulators of plant developmental processes. In this study, we demonstrate that ethylene or cGMP promote pollen tube growth in a dose-dependent manner. The etr1-1 mutant was found to be insensitive to ethylene with regard to pollen tube growth, while the growth-promoting effect of ethylene in etr2-2, ein4-4, or ein4-7 did not change, suggesting that ethylene signaling was mainly perceived by ETR1. However, the function of cGMP was not inhibited in etr1-1 and pollen tubes became insensitive to ethylene when the endogenous cGMP level was artificially decreased. This shows that cGMP is necessary for the control of pollen tube growth and that it might be a downstream component of ETR1 in the ethylene signaling pathway. Our study also found that ethylene or cGMP increase the actin bundles and elevated the percentage of relative amount of F-actin, while removal of cGMP decreased actin bundles abundance and altered the ratio of F-actin in the tip and base regions of pollen tubes. In conclusion, our data suggests that ethylene functions as the upstream signal of cGMP, and that both signals promote pollen germination and tube growth by regulating F-actin, which is essential for vesicular transport and cytoplasmic streaming.
- Subjects :
- 0301 basic medicine
Ethylene
Actin filament organization
Arabidopsis
Plant Science
Pollen Tube
Biology
medicine.disease_cause
03 medical and health sciences
chemistry.chemical_compound
Pollen
medicine
Arabidopsis thaliana
Actin
Cell Biology
General Medicine
Ethylenes
biology.organism_classification
Cell biology
Cytoplasmic streaming
Vesicular transport protein
Actin Cytoskeleton
030104 developmental biology
chemistry
Biochemistry
Pollen tube
Subjects
Details
- ISSN :
- 16156102
- Volume :
- 255
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Protoplasma
- Accession number :
- edsair.doi.dedup.....8e88864506722cfe6e6b6fb2c98ca753