Back to Search
Start Over
DNA vaccination against bovine viral diarrhoea virus induces humoral and cellular responses in cattle with evidence for protection against viral challenge
- Source :
- Vaccine. 21(17-18)
- Publication Year :
- 2003
-
Abstract
- The immune response induced by a DNA construct expressing the E2 envelope glycoprotein of bovine viral diarrhoea virus (BVDV) was studied in cattle. Four groups of five calves, were immunised by intradermal injection with a total of 1 mg of plasmid DNA on each of two occasions, with a 3-week dose interval. Group 1 received non-coding plasmid DNA only (control), group 2 received the E2 coding plasmid (0.5 mg) plus non-coding plasmid DNA (0.5 mg) and groups 3 and 4 received the E2 coding plasmid plus plasmid encoding either bovine interleukin 2 (IL-2) or granulocyte macrophage colony stimulating factor (GM-CSF) respectively. Two weeks after the final immunisation, all calves were challenged by intranasal inoculation with 5 ×10 6 TCID50 of homologous virus. On the day of challenge, neutralising antibodies were detectable in 13 of 15 vaccinated calves (one animal in each of groups 3 and 4 remained seronegative at this point). Thereafter, a strong anamnestic serological response was evident in all vaccinated animals. Furthermore, T-cell proliferation following in vitro re-stimulation with BVDV antigen was significantly elevated in the cytokine adjuvanted groups. This enhancement of BVDV specific immune responses in vaccinated animals was reflected in the clinical responses observed post-challenge. In particular, reduced febrile responses provided evidence of a disease sparing effect of vaccination. Significantly, whilst a transient viraemia was detected in all control animals following challenge, no virus was isolated from the leucocytes from 8 out of the 15 vaccinated animals. In groups 2 and 4, three animals remained virus free, although virus was isolated from two animals in each group at a single time point, while in group 3, three out of five animals had detectable viraemia. In summary, the administration of a DNA vaccine encoding only the E2 glycoprotein of BVDV induced a disease sparing effect in vaccinated calves following challenge and protected more than half of the vaccinated animals from detectable viraemia. © 2002 Elsevier Science Ltd. All rights reserved.
- Subjects :
- Cellular immunity
DNA, Complementary
Time Factors
Immunization, Secondary
Lymphocyte Activation
Virus
DNA vaccination
Body Temperature
Plasmid
Neutralization Tests
Vaccines, DNA
Animals
Cloning, Molecular
DNA Primers
Diarrhea Viruses, Bovine Viral
General Veterinary
General Immunology and Microbiology
biology
Base Sequence
Pestivirus
Public Health, Environmental and Occupational Health
Viral Vaccines
biology.organism_classification
Virology
Vaccination
Infectious Diseases
Humoral immunity
Immunology
biology.protein
Molecular Medicine
Bovine Virus Diarrhea-Mucosal Disease
Cattle
Antibody
Subjects
Details
- ISSN :
- 0264410X
- Volume :
- 21
- Issue :
- 17-18
- Database :
- OpenAIRE
- Journal :
- Vaccine
- Accession number :
- edsair.doi.dedup.....8f0fd5492fe39b5fb04501c33bf2f400