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Differential Gene Expression in Pycnoporus coccineus during Interspecific Mycelial Interactions with Different Competitors
- Source :
- Applied and Environmental Microbiology, Applied and Environmental Microbiology, American Society for Microbiology, 2013, 79 (21), pp.6626-6636. ⟨10.1128/AEM.02316-13⟩, Applied and Environmental Microbiology, 2013, 79 (21), pp.6626-6636. ⟨10.1128/AEM.02316-13⟩
- Publication Year :
- 2013
- Publisher :
- American Society for Microbiology, 2013.
-
Abstract
- Fungi compete against each other for environmental resources. These interspecific combative interactions encompass a wide range of mechanisms. In this study, we highlight the ability of the white-rot fungus Pycnoporus coccineus to quickly overgrow or replace a wide range of competitor fungi, including the gray-mold fungus Botrytis cinerea and the brown-rot fungus Coniophora puteana . To gain a better understanding of the mechanisms deployed by P. coccineus to compete against other fungi and to assess whether common pathways are used to interact with different competitors, differential gene expression in P. coccineus during cocultivation was assessed by transcriptome sequencing and confirmed by quantitative reverse transcription-PCR analysis of a set of 15 representative genes. Compared with the pure culture, 1,343 transcripts were differentially expressed in the interaction with C. puteana and 4,253 were differentially expressed in the interaction with B. cinerea , but only 197 transcripts were overexpressed in both interactions. Overall, the results suggest that a broad array of functions is necessary for P. coccineus to replace its competitors and that different responses are elicited by the two competitors, although a portion of the mechanism is common to both. However, the functions elicited by the expression of specific transcripts appear to converge toward a limited set of roles, including detoxification of secondary metabolites.
- Subjects :
- [SDV.SA]Life Sciences [q-bio]/Agricultural sciences
food.ingredient
Molecular Sequence Data
Mycology
Fungus
Pycnoporus coccineus
Applied Microbiology and Biotechnology
Fungal Proteins
03 medical and health sciences
food
Species Specificity
Gene Expression Regulation, Fungal
Botany
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Gene
030304 developmental biology
Botrytis cinerea
Botrytis
Genetics
0303 health sciences
Fungal protein
Base Sequence
Mycelium
Ecology
biology
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, RNA
030306 microbiology
Gene Expression Profiling
biology.organism_classification
Pycnoporus
Gene expression profiling
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 10985336 and 00992240
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Applied and Environmental Microbiology
- Accession number :
- edsair.doi.dedup.....8f472a6b058dc1b84b9acf0a1d96462b
- Full Text :
- https://doi.org/10.1128/aem.02316-13