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Oleanolic acid reshapes the gut microbiota and alters immune‐related gene expression of intestinal epithelial cells
- Source :
- Journal of the Science of Food and Agriculture. 102:764-773
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- BACKGROUND Oleanolic acid (OA) is a pentacyclic triterpenoid compound that is present at high levels in olive oil and has several promising pharmacological effects, such as liver protection and anti-inflammatory, antioxidant, and anticancer effects. The purpose of the present study was to assess whether OA treatment affects gut health compared to a control condition, including gut microbiota and intestinal epithelial immunity. RESULTS Illumina MiSeq sequencing (16S rRNA gene) was used to investigate the effect of OA on the microbial community of the intestinal tract, while Illumina HiSeq (RNA-seq) technology was used to investigate the regulatory effect of OA on gene expression in intestinal epithelial cells, which allowed for a comprehensive analysis of the effects of OA on intestinal health. The results showed that the consumption of OA initially controlled weight gain in mice and altered the composition of the gut microbiota. At the phylum level, OA significantly increased the relative abundances of cecum Firmicutes but decreased the abundance of Actinobacteria, and at the genus level it increased the relative abundance of potentially beneficial bacteria such as Oscillibacter and Ruminiclostridium 9. Oleanolic acid treatment also altered the expression of 12 genes involved in the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways of complement and coagulation cascades, hematopoietic cell lineage, and leukocyte transendothelial migration in intestinal epithelial cells to improve gut immunity. CONCLUSION Intake of OA can contribute beneficial effects by optimizing gut microbiota and altering the immune function of intestinal epithelial cells, potentially to improve intestinal health status. © 2021 Society of Chemical Industry.
- Subjects :
- DNA, Bacterial
Male
Firmicutes
Gene Expression
Gut flora
Cell Line
Microbiology
Mice
chemistry.chemical_compound
Cecum
Immune system
Immunity
RNA, Ribosomal, 16S
Gene expression
medicine
Animals
Humans
Intestinal Mucosa
Oleanolic Acid
Gene
Oleanolic acid
Mice, Inbred BALB C
Nutrition and Dietetics
Bacteria
biology
Epithelial Cells
biology.organism_classification
Gastrointestinal Microbiome
medicine.anatomical_structure
chemistry
Agronomy and Crop Science
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 10970010 and 00225142
- Volume :
- 102
- Database :
- OpenAIRE
- Journal :
- Journal of the Science of Food and Agriculture
- Accession number :
- edsair.doi.dedup.....8f8a0d4651af2f521cbd44d8cc5b1c6e