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Comparative assessment of three RNA extraction methods for obtaining high-quality RNA from Candida viswanathii biomass
- Source :
- Journal of microbiological methods. 184
- Publication Year :
- 2020
-
Abstract
- Isolating high quality RNA is a limiting factor in molecular analysis, since it is the base for transcriptional studies. The RNA extraction method can directly affect the RNA quality and quantity, as well as, its overall cost. The industrial importance of the yeast genus Candida in several sectors comes from their capacity to produce Lipases. These enzymes are one of the main metabolites produced by some Candida species, and it has been shown that Candida yeast can biodegrade petroleum hydrocarbons and diesel oil from biosurfactants that they can produce, a feature that turns these organisms into potential combatants for bioremediation techniques. Thus, this study aimed to determine an efficient method for isolating high quality RNA from Candida viswanathii biomass. To achieve this aim, three different RNA extraction methods, TRIzol, Hot Acid Phenol, and CTAB (Cetyltrimethylammonium Bromide), were tested. The three tested methods allowed the isolation of high-quality RNA from C. viswanathii biomass and yielded suitable RNA quantity for carrying out RT-qPCR studies. In addition, all methods displayed high sensitivity for the expression analysis of the CvGPH1 gene through RT-qPCR, with TRIzol and CTAB showing the best results and the CTAB method displaying the best cost-benefit ratio (US$0.35/sample).
- Subjects :
- Microbiology (medical)
0303 health sciences
Chromatography
biology
Phenol
030306 microbiology
Chemistry
Cetrimonium
Candida viswanathii
RNA
RNA, Fungal
Chemical Fractionation
biology.organism_classification
Microbiology
Polymerase Chain Reaction
Yeast
03 medical and health sciences
Bioremediation
Trizol
Gene expression
RNA extraction
Molecular Biology
Gene
030304 developmental biology
Candida
Subjects
Details
- ISSN :
- 18728359
- Volume :
- 184
- Database :
- OpenAIRE
- Journal :
- Journal of microbiological methods
- Accession number :
- edsair.doi.dedup.....8fbd7e86e52407fbf663727b9bd4ebde