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Optimization of Free Phytoprostane and Phytofuran Production by Enzymatic Hydrolysis of Pea Extracts Using Esterases
- Source :
- Journal of Agricultural and Food Chemistry, Journal of Agricultural and Food Chemistry, American Chemical Society, 2020, 68 (11), pp.3445-3455. ⟨10.1021/acs.jafc.9b06624⟩
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- International audience; Given the growing interest in phytoprostanes (PhytoPs) and phytofurans (PhytoFs) in the fields of plant physiology, biotechnology, and biological function, the present study aims to optimize a method of enzymatic hydrolysis that utilizes bacterial and yeast esterases that allow the appropriate quantification of PhytoPs and PhytoFs. To obtain the highest concentration of PhytoPs and PhytoFs, a response surface methodology/Box−Behnken design was used to optimize the hydrolysis conditions. Based on the information available in the literature on the most critical parameters that influence the activity of esterases, the three variables selected for the study were temperature (°C), time (min), and enzyme concentration (%). The optimal hydrolysis conditions retrieved differed between PhytoPs (21.5°C, 5.7 min, and 0.61 μg of enzyme per reaction) and PhytoFs (20.0°C, 5.0 min, and 2.17 μg of enzyme per reaction) and provided up to 25.1-and 1.7-fold higher contents relative to nonhydrolyzed extracts. The models were validated by comparing theoretical and experimental values for PhytoP and PhytoF yields (1.01 and 1.06 theoretical/experimental rates, respectively). The optimal conditions were evaluated for their relative influence on the yield of individual nonesterified PhytoPs and PhytoFs to define the limitations of the models for obtaining the highest concentration of most considered compounds. In conclusion, the models developed provided valuable alternatives to the currently applied methods using unspecific alkaline hydrolysis to obtain free nonesterified PhytoPs and PhytoFs, which give rise to more specific hydrolysis of PhytoP and PhytoF esters, reducing the degradation of free compounds by classical chemical procedures.
- Subjects :
- 0106 biological sciences
esterase
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
01 natural sciences
Esterase
response surface methodology
Hydrolysis
plant oxylipins
Enzymatic hydrolysis
Response surface methodology
Furans
Alkaline hydrolysis
chemistry.chemical_classification
Chromatography
Plant Extracts
010401 analytical chemistry
Esterases
Peas
enzymatic hydrolysis
General Chemistry
Box–Behnken design
0104 chemical sciences
Enzyme
chemistry
Yield (chemistry)
free plant oxylipins
General Agricultural and Biological Sciences
Box−Behnken
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 00218561 and 15205118
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry, Journal of Agricultural and Food Chemistry, American Chemical Society, 2020, 68 (11), pp.3445-3455. ⟨10.1021/acs.jafc.9b06624⟩
- Accession number :
- edsair.doi.dedup.....8fd8a70581a5d7818fe3994d5dac9173