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Immobilization of glucose oxidase on graphene and cobalt phthalocyanine composite and its application for the determination of glucose
- Source :
- Enzyme and microbial technology. 66
- Publication Year :
- 2014
-
Abstract
- We described a simple and facile chemical reduction strategy for the preparation of graphene (GR)-cobalt phthalocyanine (CoPc) composite and explored it for the enzymatic determination of glucose. CoPc is an active mediator and electrocatalysts for the immobilization of GOx and determination of glucose. However, it is not stable on the electrode surface and also suffers from lack of conductivity. Here, we have employed GR as the suitable support to stabilize CoPc through simple chemical reduction method and the resulting composite has been used for the glucose biosensor application. Scanning electron microscopy, X-ray diffraction and Energy-dispersive X-ray spectroscopy studies confirmed the successful formation of composite. Direct electron transfer of glucose oxidase (GOx) was observed with well defined redox peaks at the formal potential of -0.44 V. The amount of electroactive GOx (Г) and electron transfer rate constant (ks) were calculated to be 3.77×10(-10) mol cm(-2) and 3.57 s(-1), respectively. The fabricated amperometric biosensor detects glucose in wide linear concentration range from 10 μM to 14.8 mM with high sensitivity of 5.0 9μA mM(-1) cm(-2). The sensor offered very low detection limit (LOD) of 1.6 μM. In addition, practical feasibility of the sensor has been explored in screen printing carbon electrode with accurate determination of glucose present in human blood serum and urine samples. Furthermore, the sensor exhibited appreciable stability, repeatability and reproducibility results.
- Subjects :
- Blood Glucose
Materials science
Indoles
Analytical chemistry
Bioengineering
Biosensing Techniques
Urinalysis
Applied Microbiology and Biotechnology
Biochemistry
Redox
law.invention
Nanocomposites
chemistry.chemical_compound
Electron transfer
Glucose Oxidase
X-Ray Diffraction
law
Organometallic Compounds
Humans
Glucose oxidase
Detection limit
biology
Graphene
Reproducibility of Results
Spectrometry, X-Ray Emission
Enzymes, Immobilized
Amperometry
Glucose
chemistry
Dielectric Spectroscopy
Phthalocyanine
biology.protein
Microscopy, Electron, Scanning
Graphite
Biosensor
Biotechnology
Nuclear chemistry
Subjects
Details
- ISSN :
- 18790909
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Enzyme and microbial technology
- Accession number :
- edsair.doi.dedup.....902226646c83dfde14c8bcd3fc7a1492